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Fluo-4 AM

Cat. No. 6255 2 篇引用文献 2 条评论 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Cell-permeable, fluorescent Ca2+ indicator
化学名： N-[4-[6-[(Acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]glycine (acetyloxy)methyl ester
纯度： ≥95% (HPLC)
说明书
引用文献 (2)
评论 (2)
文献 (1)
生物活性 技术数据 说明书 参考文献

生物活性 for Fluo-4 AM

Fluo-4 AM is a cell-permeable, fluorescent Ca2+ indicator (Kd Ca2+ = 345 nM). Displays no resting signal and 100-fold increase in emission intensity upon Ca2+ binding. Excitation/emission λ 494/506 nm.

It is recommended to prepare stock solutions in DMSO.

Optical Data for Fluo-4 AM

Emission Color Green
λabs 494 nm
λem 506 nm
Closest Laser line 488 nm
Cell Permeable Yes
Application Fluorescence Microscopy, Flow Cytometry, Fluorometric Microplate Reading Assays

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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技术数据 for Fluo-4 AM

分子量 1096.95
公式 C51H50F2N2O23
储存 Store at -20°C
纯度 ≥95% (HPLC)
CAS Number 273221-67-3
PubChem ID 4060965
InChI Key QOMNQGZXFYNBNG-UHFFFAOYSA-N
Smiles O=C(C(F)=C1)C=C2C1=C(C3=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC4=CC(C)=CC=C4N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C3)C5=CC(F)=C(OCOC(C)=O)C=C5O2

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

产品说明书 for Fluo-4 AM

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化学品安全技术说明书

参考文献 for Fluo-4 AM

参考文献是支持产品生物活性的出版物。

Gee et al (2000) Chemical and physiological characterization of fluo-4 Ca2+-indicator dyes. Cell Calcium. 27 97 PMID: 10756976

If you know of a relevant reference for Fluo-4 AM, please let us know.

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关键词: Fluo-4 AM, Fluo-4 AM supplier, Fluo-4AM, Fluo-4, cell, permeable, calcium, Ca2+, indicator, fluorescent, Ion, Indicators, General, Calcium, Signaling, Agents, Fluorescent, 6255, Tocris Bioscience

2 篇 Fluo-4 AM 的引用文献

引用文献是使用了 Tocris 产品的出版物。 Fluo-4 AM 的部分引用包括：

Gerald W et al (2022) Norepinephrine transporter defects lead to sympathetic hyperactivity in Familial Dysautonomia models. Nat Commun 13 7032 PMID: 36396637

Bernardo et al (2020) Heterosynaptic Plasticity Determines the Set Point for Cortical Excitatory-Inhibitory Balance. Neuron 106 842-854.e4 PMID: 32213321

您是否知道使用了 Tocris Fluo-4 AM 的优秀论文？ 请告知我们.

Fluo-4 AM 的评论

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Fluo-4 detects store-operated Ca2+ entry upon ER Ca2+ store depletion in RBL-2H3 cells..
By Niroj Shrestha on 12/24/2019
分析类型： In Vitro
种属： Rat
细胞系/组织： RBL-2H3

RBL-2H3 cells cultured on glass coverslip were loaded with 2 μM Fluo-4AM in normal tyrode solution (NT) for 40 min at room temperature in the dark. Then, the cells were washed twice and left to equilibrate for at least 20 min in NT. Fluo-4-loaded cells were excited at 490 nm and fluorescence was captured at 510 nm every 2.4 s and normalized to the initial value. ER Ca2+ store was depleted with 500 nM thapsigargin which promotes store-operated Ca2+ entry into the cells upon addition of 2 mM extracellular Ca2+.

review image
works well in motor neurons.
By Karthik krishnamurthy on 10/24/2018
分析类型： In Vitro
种属： Rat
细胞系/组织： motor neurons

Used for imaging calcium entry in motor neurons at 1 mM concentration

motor neurons loaded with 1 mM concentration of fluo4-am loaded at 37 degrees for 15 minutes in aCSF. After 15 minutes cells were gently washed with aCSF twice and left in dark for 10 minutes for complete de-esterification of the dye.

review image

该领域的文献

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals