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CHIR 99021

Cat. No. 4423 412 篇引用文献 16 条评论 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
GMP Version Available: CHIR 99021
Pricing Availability   Qty
说明： Highly selective GSK-3 inhibitor; acts as Wnt activator
别名： CHIR99021,CT99021
化学名： 6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitrile
纯度： ≥98% (HPLC)
说明书
引用文献 (412)
评论 (16)
实验方案 (13)
文献 (4)
通路 (1)
生物活性 Scientific Data 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for CHIR 99021

CHIR 99021 is a potent and highly selective inhibitor of glycogen synthase kinase 3 (GSK-3) (IC50 values are 6.7 and 10 nM for GSK-3β and GSK-3α respectively). Acts as Wnt activator. Exhibits >500-fold selectivity for GSK-3 over closely related kinases; also displays >800-fold selectivity against 45 additional enzymes and receptors. In combination with the irreversible inhibitor of LSD1 (Cat. No. 3852), CHIR 99021 enables reprogramming of mouse embryonic fibroblasts, transduced by Oct4 and Klf4 only, into iPSCs. Enhances mouse and human ESC self-renewal when used in combination with PD 0325901 (Cat. No. 4192). It promotes differentiation of hPSCs in cardiomyocytes and allows formation of extended pluripotent stem (EPS) cells in combination with (S)-(+)-Dimethindene maleate (Cat. No. 1425), tetracycline antibiotic (Cat. No. 3268) and human leukemia inhibitory factor. Used in a protocol to generate brain organoids from human iPSCs. Also used in iPS cell differentiation protocol that produces CD34+ hematopoietic cells with multilineage engraftment ability.

CHIR 99021 synthesized to cGMP guidelines and CHIR 99021 in solution also available.

For more information on how to use CHIR 99021, see our protocols: Transdifferentiating Fibroblasts into Neurons, Culturing Transcriptomically Distinct Pluripotent mESCs, Converting Fibroblasts into Cardiomyocytes (9C Cocktail), Human iPSC-derived Myoblasts.

Scientific Data

Cardiomyocyte derived from human induced pluripotent stem cells (hiPSCs) using CHIR 99021. View Larger

Cardiomyocyte derived from human induced pluripotent stem cells (hiPSCs) using CHIR 99021. Cells cultured in Media supplemented with Activin A (Catalog # 338-AC, R&D Systems), N21-MAX Insulin Free (Catalog # AR010, R&D Systems), BMP-4 (Catalog # 314-BP, R&D Systems), bFGF (Catalog # 233-FB, R&D Systems) and DKK-1 (Catalog # 5439-DK, R&D Systems). Stained for F-Actin (Phalloidin, green), Troponin T (red, Human Troponin T (Cardiac) Antibody, Catalog # MAB1874, R&D Systems) and counterstained with DAPI (blue, Catalog # 5748, Tocris).

External Portal Information for CHIR 99021

Chemicalprobes.org is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of CHIR-99021 is reviewed on the chemical probes website.

化合物库 for CHIR 99021

CHIR 99021 is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library, Tocriscreen Epigenetics Library and Tocriscreen Stem Cell Library. 了解 Tocris 化合物库的更多信息。

技术数据 for CHIR 99021

分子量 465.34
公式 C22H18Cl2N8
储存 Store at -20°C
纯度 ≥98% (HPLC)
CAS Number 252917-06-9
PubChem ID 9956119
InChI Key AQGNHMOJWBZFQQ-UHFFFAOYSA-N
Smiles ClC(C=C1Cl)=CC=C1C2=NC(NCCNC3=CC=C(C#N)C=N3)=NC=C2C4=NC=C(C)N4

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for CHIR 99021

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 9.31 20

制备储备液 for CHIR 99021

以下数据基于产品分子量 465.34。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.2 mM 10.74 mL 53.72 mL 107.45 mL
1 mM 2.15 mL 10.74 mL 21.49 mL
2 mM 1.07 mL 5.37 mL 10.74 mL
10 mM 0.21 mL 1.07 mL 2.15 mL

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产品说明书 for CHIR 99021

分析证书/产品说明书
选择另一批次：
化学品安全技术说明书

参考文献 for CHIR 99021

参考文献是支持产品生物活性的出版物。

Pan et al (2011) AKT kinase activity is required for Li to modulate mood-related behaviors in mice. Neuropsychopharmacology 36 1397 PMID: 21389981

Ring et al (2003) Selective glycogen synthase kinase 3 inhibitors potentiate Ins activation of glucose transport and utilization in vitro and in vivo. Diabetes 52 588 PMID: 12606497

Li et al (2009) Generation of human-induced pluripotent stem cells in the absence of exogenous Sox2. Stem Cells 27 2992 PMID: 19839055

Ye et al (2012) Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embyronic stem cells from refractory mouse strains. PLoS One 7 e35892 PMID: 22540008

Yang et al (2017) Derivation of pluripotent stem cells with in vivo embryonic and extraembryonic potency. Cell. 169 243 PMID: 28388409

Sato et al (2015) SnapShot: Growing Organoids from Stem Cells. Cell 161 1700 PMID: 26091044

Mahe et al (2014) Establishment of gastrointestinal epithelial organoids Curr.Protoc. Mouse Biol. 3 217 PMID: 25105065

Bartfeld et al (2015) In vitro expansion of human gastric epithelial stem cells and their responses to bacterial infection. Gastroenterology 148 126 PMID: 25307862

Xia et al (2014) The generation of kidney organoids by differentiation of human pluripotent cells to ureteric bud progenitor-like cells. Nat. Protoc. 9 2693 PMID: 25340442

Dye et al (2015) In vitro generation of human pluripotent stem cell derived lung organoids. Elife PMID: 25803487

Koehler et al (2014) 3D mouse embryonic stem cell culture for generating inner ear organoids. Nat.Protoc. 9 1229 PMID: 24784820

Wimmer et al (2019) Human blood vessel organoids as a model of diabetic vasculopathy. Nature 565 505 PMID: 30651639

Noor et al (2019) 3D Printing of Personalized Thick and Perfusable Cardiac Patches and Hearts. Adv.Sci.(Weinh) 6 1900344 PMID: 31179230

Hoang et al (2018) Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells. Nat.Protoc. 13 723 PMID: 29543795

Lian et al (2012) Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc.Natl.Acad.Sci.U.S.A. 109 E1848 PMID: 22645348

Schafer et al (2023) An in vivo neuroimmune organoid model to study human microglia phenotypes. Cell 186 1222 PMID: 37172564

Ng et al (2024) Long-term engrafting multilineage hematopoietic cells differentiated from human induced pluripotent stem cells. Nat.Biotechnol. PMID: 39223325

If you know of a relevant reference for CHIR 99021, please let us know.

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关键词: CHIR 99021, CHIR 99021 supplier, CHIR99021, glycogen, synthase, kinase, 3, gsk-3, gsk-3a, gsk-3alpha, gsk-3α, gsk-3b, gsk-3beta, gsk-3β, inhibitors, inhibits, highly, selective, potent, organoids, Wnt, agonist, activator, CHIR, CT99021, CT, 99021, hematopoietic, haematopoietic, stem, cells, HSC, CD34+, multi, Glycogen, Synthase, Kinase, Stem, Cell, Reprogramming, Organoids, Hematopoietic, Cells, 4423, Tocris Bioscience

412 篇 CHIR 99021 的引用文献

引用文献是使用了 Tocris 产品的出版物。 CHIR 99021 的部分引用包括：

Muthusamy et al (2014) A method to identify and isolate pluripotent human stem cells and mouse epiblast stem cells using lipid body-associated retinyl ester fluorescence. Stem Cell Reports 3 169 PMID: 25068130

Zhu et al (2014) Mouse liver repopulation with hepatocytes generated from human fibroblasts. Nature 508 93 PMID: 24572354

Murase et al (2024) In vitro reconstitution of epigenetic reprogramming in the human germ line. Nature PMID: 38768632

Yang et al (2024) Metformin decelerates aging clock in male monkeys. Cell 187 1 PMID: 39270656

Ng et al (2024) Long-term engrafting multilineage hematopoietic cells differentiated from human induced pluripotent stem cells. Nat.Biotechnol. PMID: 39223325

Shafa et al (2018) Human-Induced Pluripotent Stem Cells Manufactured Using a Current Good Manufacturing Practice-Compliant Process Differentiate Into Clinically Relevant Cells From Three Germ Layers. Front Med (Lausanne) 5 69 PMID: 29600249

Tata et al (2018) Myoepithelial Cells of Submucosal Glands Can Function as Reserve Stem Cells to Regenerate Airways after Injury. Cell Stem Cell 22 668 PMID: 29656943

Zhou et al (2018) Functional comparison of distinct Brachyury+ states in a renal differentiation assay. Biol Open 7 PMID: 29666052

Levardon et al (2018) Expansion of Airway Basal Cells and Generation of Polarized Epithelium. Bio Protoc 8 PMID: 30009215

Shekhar et al (2018) ETV1 activates a rapid conduction transcriptional program in rodent and human cardiomyocytes. Sci Rep 8 9944 PMID: 29967479

Leung et al (2018) Notch and Aryl Hydrocarbon Receptor Signaling Impact Definitive Hematopoiesis from Human Pluripotent Stem Cells. Stem Cells PMID: 29569827

Wang et al (2018) The COPII cargo adapter SEC24C is essential for neuronal homeostasis. J Clin Invest 128 3319 PMID: 29939162

Li et al (2018) Rapid generation of gene-targeted EPS-derived mouse models through tetraploid complementation. Protein Cell PMID: 29948855

Srikanth et al (2018) Shared effects of DISC1 disruption and elevated WNT signaling in human cerebral organoids. Transl Psychiatry 8 77 PMID: 29643329

Sharma et al (2018) Stage-specific Effects of Bioactive Lipids on Human iPSC Cardiac Differentiation and Cardiomyocyte Proliferation. Sci Rep 8 6618 PMID: 29700394

Yamoah et al (2018) Highly efficient transfection of human induced pluripotent stem cells using magnetic nanoparticles. Int J Nanomedicine 13 6073 PMID: 30323594

Watmuff et al (2015) Human pluripotent stem cell derived midbrain PITX3(eGFP/w) neurons: a versatile tool for pharmacological screening and neurodegenerative modeling. Front Cell Neurosci 9 104 PMID: 25873861

Zhang et al (2015) GSK3β-Dzip1-Rab8 cascade regulates ciliogenesis after mitosis. PLoS Biol 13 e1002129 PMID: 25860027

Francipane and Lagasse (2015) The lymph node as a new site for kidney organogenesis. Stem Cells Transl Med 4 295 PMID: 25646529

Kearns et al (2015) Functional annotation of native enhancers with a Cas9-histone demethylase fusion. Nat Methods 12 401 PMID: 25775043

Jain et al (2015) Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung. Nat Commun 6 6727 PMID: 25865356

Huebsch et al (2015) Automated Video-Based Analysis of Contractility and Calcium Flux in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Cultured over Different Spatial Scales. Tissue Eng Part C Methods 21 467 PMID: 25333967

Kim et al (2021) Biphasic activation of WNT signaling facilitates the derivation of midbrain dopamine neurons from hESCs for translational use. Cell Stem Cell 28 343 PMID: 33545081

Xiong et al (2021) Human Stem Cell-Derived Neurons Repair Circuits and Restore Neural Function cell stem Cell 28 112 PMID: 32966778

Wimmer et al (2019) Human blood vessel organoids as a model of diabetic vasculopathy. Nature 565 505 PMID: 30651639

Lang et al (2019) Single-Cell Sequencing of iPSC-DA Neurons Reconstructs Disease Progression and Identifies HDAC4 as a Regulator of Parkinson Cell Phenotypes. Cell Stem Cell 24 93 PMID: 30503143

Zhang et al (2019) Implantation initiation of self-assembled embryo-like structures generated using three types of mouse blastocyst-derived stem cells. Nat Commun 10 496 PMID: 30700702

Varghese et al (2019) Fine Tuning of Hepatocyte Differentiation from Human Embryonic Stem Cells: Growth Factor vs. Small Molecule-Based Approaches. Stem Cells Int 2019 5968236 PMID: 30805010

Vila et al (2019) Quantification of human neuromuscular function through optogenetics. Theranostics 9 1232 PMID: 30867827

Yap et al (2019) In Vivo Generation of Post-infarct Human Cardiac Muscle by Laminin-Promoted Cardiovascular Progenitors. Cell Rep 26 3231 PMID: 30893597

Heuberger et al (2019) A C/EBPα-Wnt connection in gut homeostasis and carcinogenesis. Life Sci Alliance 2 e201800173 PMID: 30599048

Hiromitsu et al (2021) Generation of Functional Organs Using a Cell-Competitive Niche in Intra- and Inter-species Rodent Chimeras. Cell Stem Cell 28 141-149.e3 PMID: 33373620

Bianca et al (2021) Prednisolone rescues Duchenne muscular dystrophy phenotypes in human pluripotent stem cell-derived skeletal muscle in vitro. Proc Natl Acad Sci U S A 118 PMID: 34260377

Ari et al (2021) NOTUM from Apc-mutant cells biases clonal competition to initiate cancer. Nature 594 430-435 PMID: 34079124

Madeline A et al (2021) Generation and long-term culture of advanced cerebral organoids for studying later stages of neural development. Nat Protoc 16 579-602 PMID: 33328611

Beth Y et al (2021) Human iPSC-derived fallopian tube organoids with BRCA1 mutation recapitulate early-stage carcinogenesis. Cell Rep 37 110146 PMID: 34965417

Karen E et al (2021) Direct SARS-CoV-2 infection of the human inner ear may underlie COVID-19-associated audiovestibular dysfunction. Commun Med (Lond) 1 44 PMID: 34870285

Yannick D et al (2021) Pharmacological targeting of Sam68 functions in colorectal cancer stem cells. iScience 24 103442 PMID: 34877499

Jacco et al (2021) Generation of mixed murine organoids to model cellular interactions. STAR Protoc 2 100997 PMID: 34917977

Ludo et al (2021) Induced pluripotent stem cell-derived motor neurons of CMT type 2 patients reveal progressive mitochondrial dysfunction. Brain 144 2471-2485 PMID: 34128983

Ida et al (2021) Miniaturized 3D bone marrow tissue model to assess response to Thrombopoietin-receptor agonists in patients. Elife 10 PMID: 34059198

Jacco et al (2021) Active elimination of intestinal cells drives oncogenic growth in organoids. Cell Rep 36 109307 PMID: 34233177

Anne G et al (2021) NAD+ enhances ribitol and ribose rescue of α-dystroglycan functional glycosylation in human FKRP-mutant myotubes. Elife 10 PMID: 33513091

Ling et al (2021) Evolutionary epigenomic analyses in mammalian early embryos reveal species-specific innovations and conserved principles of imprinting. Sci Adv 7 eabi6178 PMID: 34818044

Danny et al (2021) NRF1 association with AUTS2-Polycomb mediates specific gene activation in the brain. Mol Cell 81 4663-4676.e8 PMID: 34637754

Ruili et al (2021) Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small Molecules. Methods Mol Biol 2454 811-827 PMID: 34128205

Arun et al (2021) Responsiveness to perturbations is a hallmark of transcription factors that maintain cell identity in vitro. Cell Syst 12 885-899.e8 PMID: 34352221

Rafael et al (2021) Neuronal enhancers are hotspots for DNA single-strand break repair. Nature 593 440-444 PMID: 33767446

Fiachra H. et al (2021) A diamidobenzimidazole STING agonist protects against SARS-CoV-2 infection. Sci Immunol 6 PMID: 34010139

Regent et al (2019) Automation of human pluripotent stem cell differentiation toward retinal pigment epithelial cells for large-scale productions. Sci Rep 9 10646 PMID: 31337830

Suzuki et al (2019) Directed differentiation of human induced pluripotent stem cells into mature stratified bladder urothelium. Sci Rep 9 10506 PMID: 31324820

Garcia et al (2019) Huntington's Disease Patient-Derived Astrocytes Display Electrophysiological Impairments and Reduced Neuronal Support. Front Neurosci 13 669 PMID: 31316341

Chiu et al (2019) NMDAR-Activated PP1 Dephosphorylates GluN2B to Modulate NMDAR Synaptic Content. Cell Rep 28 332 PMID: 31291571

Canizo et al (2019) A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos. BMC Dev Biol 19 13 PMID: 31272387

Xu et al (2019) Generation of pig induced pluripotent stem cells using an extended pluripotent stem cell culture system. Stem Cell Res Ther 10 193 PMID: 31248457

Frith and Tsakiridis (2019) Efficient Generation of Trunk Neural Crest and Sympathetic Neurons from Human Pluripotent Stem Cells Via a Neuromesodermal Axial Progenitor Intermediate. Curr Protoc Stem Cell Biol e81 PMID: 30688409

Du et al (2019) Efficient derivation of extended pluripotent stem cells from NOD-scid Il2rg-/- mice. Protein Cell 10 31 PMID: 29948854

Yin et al (2019) Chemical conversion of human fetal astrocytes into neurons through modulation of multiple signaling pathways. Stem Cell Reports 12 488 PMID: 30745031

Yang (2017) Mutual reinforcement between telomere capping and canonical Wnt signalling in the intestinalstem cell niche. Nat Commun 8 14766 PMID: 28303901

Yang et al (2017) Derivation of pluripotent stem cells with in vivo embryonic and extraembryonic potency. Cell. 169 243 PMID: 28388409

Xi (2017) In Vivo Human Somitogenesis Guides Somite Development from hPSCs. Cell Rep. 18 1573 PMID: 28178531

Kotton et al (2017) Differentiation of human pluripotent stem cells into functional lung alveolar epithelial cells. Cell Stem Cell. 21 472 PMID: 28965766

Tchieu et al (2017) A modular platform for differentiation of human PSCs into all major ectodermal lineages. Cell Stem Cell. 21 399 PMID: 28886367

Shao (2017) A pluripotent stem cell-based model for post-implantation human amniotic sac development. Nat Commun 8 208 PMID: 28785084

Brown (2017) Live imaging reveals that the first division of differentiating human embryonic stem cells often yields asymmetric fates. Cell Rep 21 301 PMID: 29020617

Mitchell J et al (2014) Hematopoietic differentiation of pluripotent stem cells in culture. Methods Mol Biol 1185 181-94 PMID: 25062629

Yoon-Young et al (2014) Determination of Functional Activity of Human iPSC-Derived Hepatocytes by Measurement of CYP Metabolism. Methods Mol Biol 1357 383-94 PMID: 25410290

Kenneth et al (2014) Transcriptional profiling of Wnt3a mutants identifies Sp transcription factors as essential effectors of the Wnt/β-catenin pathway in neuromesodermal stem cells. PLoS One 9 e87018 PMID: 24475213

Yoon-Young et al (2014) In Vitro Modeling of Alcohol-Induced Liver Injury Using Human-Induced Pluripotent Stem Cells. Methods Mol Biol 1353 271-83 PMID: 25520290

Peter et al (2014) Generation, expansion and functional analysis of endothelial cells and pericytes derived from human pluripotent stem cells. Nat Protoc 9 1514-31 PMID: 24874816

Lorenz et al (2013) Dual-SMAD Inhibition/WNT Activation-Based Methods to Induce Neural Crest and Derivatives from Human Pluripotent Stem Cells. Methods Mol Biol 1307 329-43 PMID: 24301074

Ying et al (2021) Airway basal stem cells generate distinct subpopulations of PNECs. Cell Rep 35 109011 PMID: 33882306

Li et al (2021) 5-hydroxymethylcytosine is dynamically regulated during forebrain organoid development and aberrantly altered in Alzheimer's disease. Cell Rep 35 109042 PMID: 33910000

Anestis et al (2021) Generating Enteric Nervous System Progenitors from Human Pluripotent Stem Cells. Curr Protoc 1 e137 PMID: 34102038

Wei et al (2021) Generation of human blastocyst-like structures from pluripotent stem cells. Cell Discov 7 81 PMID: 34489415

Brian J et al (2021) A high-content platform for physiological profiling and unbiased classification of individual neurons. Cell Rep Methods 1 PMID: 34318289

Andrea et al (2021) Evaluating totipotency using criteria of increasing stringency. Nat Cell Biol 23 49-60 PMID: 33420491

Digvijay et al (2021) HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells. Science 371 PMID: 33335017

Marcela et al (2021) Generation of functional ciliated cholangiocytes from human pluripotent stem cells. Nat Commun 12 6504 PMID: 34764255

Anestis et al (2021) In Vitro Generation of Posterior Motor Neurons from Human Pluripotent Stem Cells. Curr Protoc 1 e244 PMID: 34547185

John D et al (2021) Elevated Extracellular cGMP Produced after Exposure to Enterotoxigenic Escherichia coli Heat-Stable Toxin Induces Epithelial IL-33 Release and Alters Intestinal Immunity. Infect Immun 89 PMID: 33431701

Yi et al (2021) The histone demethylase KDM2B regulates human primordial germ cell-like cells specification. Int J Biol Sci 17 527-538 PMID: 33613110

Konstantinos-Dionysios et al (2021) Sequence logic at enhancers governs a dual mechanism of endodermal organ fate induction by FOXA pioneer factors. Nat Commun 12 6636 PMID: 34789735

Takuya et al (2021) GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program. Life Sci Alliance 4 PMID: 33608411

Jason G et al (2021) Robust single-cell discovery of RNA targets of RNA-binding proteins and ribosomes. Nat Methods 18 507-519 PMID: 33963355

Ulrich et al (2021) An early cell shape transition drives evolutionary expansion of the human forebrain. Cell 184 2084-2102.e19 PMID: 33765444

Gustavo et al (2021) Human Pluripotent Stem Cell-Derived Intestinal Organoids Model SARS-CoV-2 Infection Revealing a Common Epithelial Inflammatory Response. Stem Cell Reports 16 940-953 PMID: 33852884

John M et al (2021) A weakened interface in the P182L variant of HSP27 associated with severe Charcot-Marie-Tooth neuropathy causes aberrant binding to interacting proteins. EMBO J 40 e103811 PMID: 33644875

Su-Jin et al (2021) Bioactive Lipid O-cyclic phytosphingosine-1-phosphate Promotes Differentiation of Human Embryonic Stem Cells into Cardiomyocytes via ALK3/BMPR Signaling. Int J Mol Sci 22 PMID: 34209900

David et al (2021) Mitochondrial bioenergetic deficits in C9orf72 amyotrophic lateral sclerosis motor neurons cause dysfunctional axonal homeostasis. Acta Neuropathol 141 257-279 PMID: 33398403

Anne G et al (2021) A universal gene correction approach for FKRP-associated dystroglycanopathies to enable autologous cell therapy. Cell Rep 36 109360 PMID: 34260922

Ha Neui et al (2021) The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury. Neurobiol Dis 152 105294 PMID: 33549720

Paul et al (2021) Sarcomere function activates a p53-dependent DNA damage response that promotes polyploidization and limits in vivo cell engraftment. Cell Rep 35 109088 PMID: 33951429

Andrew et al (2021) Patient-specific iPSCs carrying an SFTPC mutation reveal the intrinsic alveolar epithelial dysfunction at the inception of interstitial lung disease. Cell Rep 36 109636 PMID: 34469722

Giovanni et al (2021) CHAF1A Blocks Neuronal Differentiation and Promotes Neuroblastoma Oncogenesis via Metabolic Reprogramming. Adv Sci (Weinh) 8 e2005047 PMID: 34365742

Michael et al (2021) Pharmacological Manipulation of Wnt/β-Catenin Signaling Pathway in Human Neural Precursor Cells Alters Their Differentiation Potential and Neuronal Yield. Front Mol Neurosci 14 680018 PMID: 34421534

Bin et al (2021) Myogenin suppresses apoptosis induced by angiotensin II in human induced pluripotent stem cell-derived cardiomyocytes. Biochem Biophys Res Commun 552 84-90 PMID: 33743352

Philip J et al (2021) Transplantable human motor networks as a neuron-directed strategy for spinal cord injury. iScience 24 102827 PMID: 34381965

Paolo et al (2021) Paediatric gastric organoids as a tool for disease modelling and clinical translation. Pediatr Surg Int 37 317-324 PMID: 33495862

Xinwen et al (2021) Host metabolism dysregulation and cell tropism identification in human airway and alveolar organoids upon SARS-CoV-2 infection. Protein Cell 12 717-733 PMID: 33314005

Kyoung-Jae et al (2021) TENET: gene network reconstruction using transfer entropy reveals key regulatory factors from single cell transcriptomic data. Nucleic Acids Res 49 e1 PMID: 33170214

Won Kyu et al (2021) Phenotypic plasticity underlies local invasion and distant metastasis in colon cancer. Elife 10 PMID: 34036938

Di et al (2021) High-Yield Human Induced Pluripotent Stem Cell-Derived Monocytes and Macrophages Are Functionally Comparable With Primary Cells. Front Cell Dev Biol 9 656867 PMID: 33937256

Tim et al (2021) High-throughput generation of midbrain dopaminergic neuron organoids from reporter human pluripotent stem cells. STAR Protoc 2 100463 PMID: 33997803

Jamie A et al (2021) Cell Surface Mechanics Gate Embryonic Stem Cell Differentiation. Cell Stem Cell 28 209-216.e4 PMID: 33207217

Gustavo et al (2021) Expression of HIV-1 Intron-Containing RNA in Microglia Induces Inflammatory Responses. J Virol 95 PMID: 33298546

Deborah A et al (2021) Automating Human Induced Pluripotent Stem Cell Culture and Differentiation of iPSC-Derived Retinal Pigment Epithelium for Personalized Drug Testing. SLAS Technol 26 287-299 PMID: 33292045

Beth A et al (2021) Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging. Commun Biol 4 786 PMID: 34168275

Adrian S et al (2021) The miR-199a/214 Cluster Controls Nephrogenesis and Vascularization in a Human Embryonic Stem Cell Model. Stem Cell Reports 16 134-148 PMID: 33306987

Bruce R et al (2021) Bioengineered optogenetic model of human neuromuscular junction. Biomaterials 276 121033 PMID: 34403849

Alfonso Martinez et al (2021) In vitro teratogenicity testing using a 3D, embryo-like gastruloid system. Reprod Toxicol 105 72-90 PMID: 34425190

Boyang et al (2021) Generation of mouse-human chimeric embryos. Nat Protoc 16 3954-3980 PMID: 34215863

Kolja et al (2021) Local non-pituitary growth hormone is induced with aging and facilitates epithelial damage. Cell Rep 37 110068 PMID: 34910915

Yun et al (2021) Mechanical stimulation enhances development of scaffold-free, 3D-printed, engineered heart tissue grafts. J Tissue Eng Regen Med 15 503-512 PMID: 33749089

Qian et al (2021) Characterization of primary models of human trophoblast. Development 148 PMID: 34651188

Alexander et al (2021) Generation of Mouse Pluripotent Stem Cell-derived Trunk-like Structures: An in vitro Model of Post-implantation Embryogenesis. Bio Protoc 11 e4042 PMID: 34250208

Britta et al (2021) Wnt signaling mediates acquisition of blood-brain barrier properties in naïve endothelium derived from human pluripotent stem cells. Elife 10 PMID: 34755601

Juan Carlos et al (2021) Chimeric contribution of human extended pluripotent stem cells to monkey embryos ex vivo. Cell 184 2020-2032.e14 PMID: 33861963

Shingo et al (2021) Differentiation of human pluripotent stem cells into functional airway basal stem cells. STAR Protoc 2 100683 PMID: 34355203

Marcos et al (2021) The connectome of neural crest enhancers reveals regulatory features of signaling systems. Dev Cell 56 1268-1282.e6 PMID: 33852891

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Alessandro et al (2023) Establishment of Skeletal Myogenic Progenitors from Non-Human Primate Induced Pluripotent Stem Cells. Cells 12 PMID: 37190056

Tim et al (2023) A pesticide and iPSC dopaminergic neuron screen identifies and classifies Parkinson-relevant pesticides. Nat Commun 14 2803 PMID: 37193692

Jing et al (2023) Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs. NPJ Regen Med 8 26 PMID: 37236990

Aditya et al (2023) Systematic discovery of transcription factors that improve hPSC-derived cardiomyocyte maturation via temporal analysis of bioengineered cardiac tissues. APL Bioeng 7 026109 PMID: 37252678

Alessandra et al (2023) Recapitulating porcine cardiac development in vitro: from expanded potential stem cell to embryo culture models. Front Cell Dev Biol 11 1111684 PMID: 37261075

Bouffi et al (2023) In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice Nat.Biotechnol. PMID: 36702898

Schafer et al (2023) An in vivo neuroimmune organoid model to study human microglia phenotypes. Cell 186 2111 PMID: 37172564

Amos et al (2020) SEM/FIB Imaging for Studying Neural Interfaces. Dev Neurobiol 80 305-315 PMID: 31228876

Narutoshi et al (2020) Principles of Spheroid Preparation for Creation of 3D Cardiac Tissue Using Biomaterial-Free Bioprinting. Methods Mol Biol 2140 183-197 PMID: 32207113

Krisztina et al (2020) Gastruloids: Embryonic Organoids from Mouse Embryonic Stem Cells to Study Patterning and Development in Early Mammalian Embryos. Methods Mol Biol 2258 131-147 PMID: 33340359

Stewart A et al (2020) Protocol for Tri-culture of hiPSC-Derived Neurons, Astrocytes, and Microglia. STAR Protoc 1 100190 PMID: 33377084

Takuya et al (2020) Long-term expansion with germline potential of human primordial germ cell-like cells in vitro. EMBO J 39 e104929 PMID: 32954504

Mark et al (2020) Reengineering an Antiarrhythmic Drug Using Patient hiPSC Cardiomyocytes to Improve Therapeutic Potential and Reduce Toxicity. Cell Stem Cell 27 813-821.e6 PMID: 32931730

Masayuki et al (2020) Intracellular pH controls WNT downstream of glycolysis in amniote embryos. Nature 584 98-101 PMID: 32581357

Alexander R et al (2020) Spatially controlled stem cell differentiation via morphogen gradients: A comparison of static and dynamic microfluidic platforms. J Vac Sci Technol A 38 033205 PMID: 32255900

Michael A et al (2020) Differentiation of PTH-Expressing Cells From Human Pluripotent Stem Cells. Endocrinology 161 PMID: 32810225

Joshy et al (2020) Poison Exon Splicing Regulates a Coordinated Network of SR Protein Expression during Differentiation and Tumorigenesis. Mol Cell 80 648-665.e9 PMID: 33176162

Emanuela et al (2020) Differentiation of the human PAX7-positive myogenic precursors/satellite cell lineage in vitro. Development PMID: 34004948

Maxime M et al (2020) Pluripotent stem cell derived intestinal organoids with an enteric nervous system. Methods Cell Biol 159 175-199 PMID: 32586442

Kotaro et al (2020) Generation of human oogonia from induced pluripotent stem cells in culture. Nat Protoc 15 1560-1583 PMID: 32231324

Nicholas C et al (2020) Co-Culture System of Human Enteroids/Colonoids with Innate Immune Cells. Curr Protoc Immunol 131 e113 PMID: 33166041

Lucy et al (2020) Establishment and differentiation of long-term trophoblast organoid cultures from the human placenta. Nat Protoc 15 3441-3463 PMID: 32908314

Donna L et al (2020) SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids. Cell Stem Cell 27 951-961.e5 PMID: 33113348

Hyun Jung et al (2020) Neural Transplants From Human Induced Pluripotent Stem Cells Rescue the Pathology and Behavioral Defects in a Rodent Model of Huntington's Disease. Front Neurosci 14 558204 PMID: 33071737

Thaddeus G et al (2020) Placenta-derived macaque trophoblast stem cells: differentiation to syncytiotrophoblasts and extravillous trophoblasts reveals phenotypic reprogramming. Sci Rep 10 19159 PMID: 33154556

Lisa M et al (2020) FOXO3 targets are reprogrammed as Huntington's disease neural cells and striatal neurons face senescence with p16INK4a increase. Aging Cell 19 e13226 PMID: 33156570

Alon et al (2020) Electrophysiologic Characterization of Developing Human Embryonic Stem Cell-Derived Photoreceptor Precursors. Invest Ophthalmol Vis Sci 61 44 PMID: 32991686

Richard A et al (2020) Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development. Cell Stem Cell 27 822-839.e8 PMID: 32946788

Malcolm G et al (2020) Aspirin Rescues Wnt-Driven Stem-like Phenotype in Human Intestinal Organoids and Increases the Wnt Antagonist Dickkopf-1. Cell Mol Gastroenterol Hepatol 11 465-489 PMID: 32971322

Walter C et al (2020) Accelerated differentiation of human pluripotent stem cells into neural lineages via an early intermediate ectoderm population. Stem Cells 38 1400-1408 PMID: 32745311

Ludovic et al (2020) Naive Pluripotent Stem Cells Exhibit Phenotypic Variability that Is Driven by Genetic Variation. Cell Stem Cell 27 470-481.e6 PMID: 32795399

Adrian S et al (2020) Aberrant Differentiation of Human Pluripotent Stem Cell-Derived Kidney Precursor Cells inside Mouse Vascularized Bioreactors. Nephron 144 509-524 PMID: 32756058

Frédéric H-T et al (2020) Aberrant interaction of FUS with the U1 snRNA provides a molecular mechanism of FUS induced amyotrophic lateral sclerosis. Nat Commun 11 6341 PMID: 33311468

April D et al (2020) Generation of PAX7 Reporter Cells to Investigate Skeletal Myogenesis from Human Pluripotent Stem Cells. STAR Protoc 1 100158 PMID: 33377052

Emanuela et al (2020) Differentiation of the human PAX7-positive myogenic precursors/satellite cell lineage in vitro. Development 147 PMID: 32541004

Constanze et al (2020) Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells. STAR Protoc 1 100130 PMID: 33377024

Koen C et al (2020) Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids. STAR Protoc 1 100126 PMID: 33377020

Edwin M et al (2020) Human iPSC Modeling Reveals Mutation-Specific Responses to Gene Therapy in a Genotypically Diverse Dominant Maculopathy. Am J Hum Genet 107 278-292 PMID: 32707085

Takashi et al (2020) Hedgehog Activation Regulates Human Osteoblastogenesis. Stem Cell Reports 15 125-139 PMID: 32531191

Peter W et al (2020) Nucleosides Rescue Replication-Mediated Genome Instability of Human Pluripotent Stem Cells. Stem Cell Reports 14 1009-1017 PMID: 32413278

Jizhong et al (2020) Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions. STAR Protoc 1 PMID: 32734277

Mary Lou et al (2020) SARS-CoV-2 Infection of Pluripotent Stem Cell-Derived Human Lung Alveolar Type 2 Cells Elicits a Rapid Epithelial-Intrinsic Inflammatory Response. Cell Stem Cell 27 962-973.e7 PMID: 32979316

Peter et al (2020) Evaluating the Effects of Cell Sorting on Gene Expression. J Biomol Tech 31 100-111 PMID: 32982601

Rui et al (2020) Wnt/Beta-catenin/Esrrb signalling controls the tissue-scale reorganization and maintenance of the pluripotent lineage during murine embryonic diapause. Nat Commun 11 5499 PMID: 33127892

Ting et al (2020) Protocol for the Generation of Human Pluripotent Reporter Cell Lines Using CRISPR/Cas9. STAR Protoc 1 PMID: 33073252

Gareth et al (2020) A method for differentiating human induced pluripotent stem cells toward functional cardiomyocytes in 96-well microplates. Sci Rep 10 18498 PMID: 33116175

Hui et al (2020) 16p11.2 microdeletion imparts transcriptional alterations in human iPSC-derived models of early neural development. Elife 9 PMID: 33169669

Byeong-Sun et al (2020) Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2. Cell Stem Cell 27 905-919.e10 PMID: 33142113

Richard C et al (2020) Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction. Cell Stem Cell 27 890-904.e8 PMID: 33128895

Darrell N et al (2020) Generation of mesenchyme free intestinal organoids from human induced pluripotent stem cells. Nat Commun 11 215 PMID: 31924806

Qianqian et al (2020) A cytokine screen using CRISPR-Cas9 knock-in reporter pig iPS cells reveals that Activin A regulates NANOG. Stem Cell Res Ther 11 67 PMID: 32070424

Preethi et al (2020) High-Throughput Drug Screening Identifies a Potent Wnt Inhibitor that Promotes Airway Basal Stem Cell Homeostasis. Cell Rep 30 2055-2064.e5 PMID: 32075752

Sun et al (2020) Development of genetic quality tests for good manufacturing practice-compliant induced pluripotent stem cells and their derivatives. Sci Rep 10 3939 PMID: 32127560

Jeong-Eun et al (2020) Multimodal Therapeutic Effects of Neural Precursor Cells Derived from Human-Induced Pluripotent Stem Cells through Episomal Plasmid-Based Reprogramming in a Rodent Model of Ischemic Stroke. Stem Cells Int 2020 4061516 PMID: 32269595

Robert et al (2020) CRISPR-mediated gene correction links the ATP7A M1311V mutations with amyotrophic lateral sclerosis pathogenesis in one individual. Commun Biol 3 33 PMID: 31959876

Xavier et al (2020) BCL-xL/BCL2L1 is a critical anti-apoptotic protein that promotes the survival of differentiating pancreatic cells from human pluripotent stem cells. Cell Death Dis 11 378 PMID: 32424151

Constanze et al (2020) Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells. Cell Rep 31 107691 PMID: 32460028

Peter et al (2020) Evaluating the Effects of Cell Sorting on Gene Expression. J Biomol Tech PMID: 32831654

Paul et al (2020) A Non-Coding Disease Modifier of Pancreatic Agenesis Identified by Genetic Correction in a Patient-Derived iPSC Line. Cell Stem Cell 27 137-146.e6 PMID: 32442395

Katja et al (2020) A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells. Cell Stem Cell 27 158-176.e10 PMID: 32396864

Da-Zhi et al (2020) Transient exposure to miR-203 enhances the differentiation capacity of established pluripotent stem cells. EMBO J 39 e104324 PMID: 32614092

Valentina et al (2020) Intravital three-dimensional bioprinting. Nat Biomed Eng 4 901-915 PMID: 32572195

Yanyan et al (2020) Autologous correction in patient induced pluripotent stem cell-endothelial cells to identify a novel pathogenic mutation of hereditary hemorrhagic telangiectasia. Pulm Circ 10 2045894019885357 PMID: 33282178

Oliver et al (2020) Inhibition of LTβR signalling activates WNT-induced regeneration in lung. Nature 588 151-156 PMID: 33149305

Tina et al (2020) An in vitro model of early anteroposterior organization during human development. Nature 582 410-415 PMID: 32528178

Christoph et al (2020) In vitro characterization of the human segmentation clock. Nature 580 113-118 PMID: 31915384

Yuka et al (2020) Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells. Nat Commun 11 5656 PMID: 33168808

Claudio J et al (2020) Regulation of Tumor Initiation by the Mitochondrial Pyruvate Carrier. Cell Metab 31 284-300.e7 PMID: 31813825

Melissa J et al (2020) Genome Editing-Mediated Utrophin Upregulation in Duchenne Muscular Dystrophy Stem Cells. Mol Ther Nucleic Acids 22 500-509 PMID: 33230452

Jason et al (2020) Generation and trapping of a mesoderm biased state of human pluripotency. Nat Commun 11 4989 PMID: 33020476

Sheng et al (2020) Overcoming Wnt-β-catenin dependent anticancer therapy resistance in leukaemia stem cells. Nat Cell Biol 22 689-700 PMID: 32313104

Olivier et al (2020) Bioelectrical domain walls in homogeneous tissues. Nat Phys 16 357-364 PMID: 33790984

Lorna et al (2020) Differentiation of a Contractile, Ureter-Like Tissue, from Embryonic Stem Cell-Derived Ureteric Bud and Ex Fetu Mesenchyme. J Am Soc Nephrol 31 2253-2262 PMID: 32826325

Carissa et al (2020) Potently Cytotoxic Natural Killer Cells Initially Emerge from Erythro-Myeloid Progenitors during Mammalian Development. Dev Cell 53 229-239.e7 PMID: 32197069

Maija L et al (2020) Elevated de novo protein synthesis in FMRP-deficient human neurons and its correction by metformin treatment. Mol Autism 11 41 PMID: 32460900

Minhyung et al (2020) Neuroprotective Effects of Cryptotanshinone in a Direct Reprogramming Model of Parkinson's Disease. Molecules 25 PMID: 32784741

Janghwan et al (2020) The combination of forskolin and VPA increases gene expression efficiency to the hypoxia/neuron-specific system. Ann Transl Med 8 933 PMID: 32953733

Lee J et al (2020) Human Motor Neurons With SOD1-G93A Mutation Generated From CRISPR/Cas9 Gene-Edited iPSCs Develop Pathological Features of Amyotrophic Lateral Sclerosis. Front Cell Neurosci 14 604171 PMID: 33328898

Micha et al (2020) Nucleus size and DNA accessibility are linked to the regulation of paraspeckle formation in cellular differentiation. BMC Biol 18 42 PMID: 32321486

Chan Young et al (2020) Robust and Reproducible Generation of Induced Neural Stem Cells from Human Somatic Cells by Defined Factors. Int J Stem Cells 13 80-92 PMID: 32114739

Joshua M et al (2020) A novel cardiomyogenic role for Isl1+ neural crest cells in the inflow tract. Sci Adv 6 PMID: 33268364

Mustafa K et al (2020) Disrupted ER membrane protein complex-mediated topogenesis drives congenital neural crest defects. J Clin Invest 130 813-826 PMID: 31904590

Yu-Hwai et al (2020) Differentiation of Human Intestinal Organoids with Endogenous Vascular Endothelial Cells. Dev Cell 54 516-528.e7 PMID: 32841595

Jianming et al (2020) Human Bocavirus 1 Infection of Well-Differentiated Human Airway Epithelium. Curr Protoc Microbiol 58 e107 PMID: 32639683

Steven C et al (2020) Mapping the Effects of Genetic Variation on Chromatin State and Gene Expression Reveals Loci That Control Ground State Pluripotency. Cell Stem Cell 27 459-469.e8 PMID: 32795400

Vijay Pratap et al (2020) Persistent DNA Damage and Senescence in the Placenta Impacts Developmental Outcomes of Embryos. Dev Cell 54 333-347.e7 PMID: 32800293

Andrew et al (2020) Organoids Model Transcriptional Hallmarks of Oncogenic KRAS Activation in Lung Epithelial Progenitor Cells. Cell Stem Cell 27 663-678.e8 PMID: 32891189

Igor I et al (2020) Generation of T cells from Human and Nonhuman Primate Pluripotent Stem Cells. Bio Protoc 10 e3675 PMID: 33659345

Vermilyea et al (2017) Induced Pluripotent Stem Cell-Derived Dopaminergic Neurons from Adult Common Marmoset Fibroblasts. Stem Cells Dev 26 1225 PMID: 28635509

Sampaziotis et al (2017) Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells. Nat Protoc 12 814 PMID: 28333915

McCauley et al (2017) Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling. Cell Stem Cell 20 844 PMID: 28366587

Lancaster et al (2017) Guided self-organization and cortical plate formation in human brain organoids. Nat Biotechnol 35 659 PMID: 28562594

Morizane and Bonventre (2017) Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells. Nat Protoc 12 195 PMID: 28005067

Yang et al (2017) Establishment of mouse expanded potential stem cells. Nature 550 393 PMID: 29019987

Ong et al (2017) Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes. Sci Rep 7 4566 PMID: 28676704

Kumar et al (2017) Dynamic regulation of Nanog and stem cell-signaling pathways by Hoxa1 during early neuro-ectodermal differentiation of ES cells. Proc Natl Acad Sci U S A 114 5838 PMID: 28584089

Lai et al (2017) Correction of Hirschsprung-Associated Mutations in Human Induced Pluripotent Stem Cells Via Clustered Regularly Interspaced Short Palindromic Repeats/Cas9, Restores Neural Crest Cell Function. Gastroenterology 153 139 PMID: 28342760

Lee et al (2017) Anatomically and Functionally Distinct Lung Mesenchymal Populations Marked by Lgr5 and Lgr6. Cell 170 1149 PMID: 28886383

Lin et al (2017) Culture in Glucose-Depleted Medium Supplemented with Fatty Acid and 3,3',5-Triiodo-l-Thyronine Facilitates Purification and Maturation of Human Pluripotent Stem Cell-Derived Cardiomyocytes. Front Endocrinol (Lausanne) 8 253 PMID: 29067001

Tang and Cheng (2017) Cocktail of chemical compounds robustly promoting cell reprogramming protects liver against acute injury. Protein Cell 8 273 PMID: 28190217

Walsh et al (2017) Defined Culture Conditions Accelerate Small-molecule-assisted Neural Induction for the Production of Neural Progenitors from Human-induced Pluripotent Stem Cells. Cell Transplant 26 1890 PMID: 29390875

Zhang et al (2016) Pharmacological reprogramming of fibroblasts into neural stem cells by signaling-directed transcriptional activation. Cell Stem Cell 18 653 PMID: 27133794

Mou et al (2016) Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells Cell: Stem Cell 19 217 PMID: 27320041

Lu et al (2016) Generation of serotonin neurons from human pluripotent stem cells. Nat Biotechnol 34 89 PMID: 26655496

Solari et al (2015) Manganese Superoxide Dismutase Gene Expression Is Induced by Nanog and Oct4, Essential Pluripotent Stem Cells' Transcription Factors. Mol Syst Biol 10 e0144336 PMID: 26642061

Mutze et al (2015) Enolase 1 (ENO1) and protein disulfide-isomerase associated 3 (PDIA3) regulate Wnt/β-catenin-driven trans-differentiation of murine alveolar epithelial cells. Cell Rep 8 877 PMID: 26035385

Srikanth et al (2015) Genomic DISC1 Disruption in hiPSCs Alters Wnt Signaling and Neural Cell Fate. Sci Rep 12 1414 PMID: 26299970

Morizane et al (2015) Nephron organoids derived from human pluripotent stem cells model kidney development and injury. Stem Cell Reports 33 1193 PMID: 26458176

Nostro et al (2015) Efficient generation of NKX6-1+ pancreatic progenitors from multiple human pluripotent stem cell lines. Eur J Immunol 4 591 PMID: 25843049

Hill et al (2015) Glycogen synthase kinase-3 controls IL-10 expression in CD4(+) effector T-cell subsets through epigenetic modification of the IL-10 promoter. Mol Neurobiol 45 1103 PMID: 25627813

Ooi et al (2015) Inhibition of Excessive Monoamine Oxidase A/B Activity Protects Against Stress-induced Neuronal Death in Huntington Disease. Nat Chem Biol 52 1850 PMID: 25398695

Thorne et al (2015) GSK-3 modulates cellular responses to a broad spectrum of kinase inhibitors. Mol Biol Cell 11 58 PMID: 25402767

Barbosa et al (2015) Phosphorylation and SCF-mediated degradation regulate CREB-H transcription of metabolic targets. Stem Cell Reports 26 2939 PMID: 26108621

Du et al (2015) Generation and expansion of highly pure motor neuron progenitors from human pluripotent stem cells. Nat.Commun. 6 6626 PMID: 25806427

Sladitschek and Neveu (2015) The bimodally expressed microRNA miR-142 gates exit from pluripotency. J Biol Chem 11 850 PMID: 26690966

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IPS culture.
By Murali K Yanda on 09/27/2022
分析类型： In Vitro
种属： Mouse
细胞系/组织： ips

Mouse ips cells culture with 2i media.

review image
2i medium culture.
By Yu Chen on 07/11/2022
分析类型： In Vitro
种属： Mouse
细胞系/组织： ESC

ESC 2i medium culture; 3 uM

review image
ESC culture.
By Xiaoyu Zhang on 11/23/2021
分析类型： In Vitro
种属： Mouse
细胞系/组织： ESC

In vitro cell culture addition. The image shows the morphology of ESC colonies after conversion using the treatment condition. This condition supported the conversion and long-term maintenance of dome-shaped ESCs from primed ESCs.

review image
Differentiation to cartilage.
By Julieta O'Flaherty on 12/08/2020
分析类型： In Vitro
种属： Human
细胞系/组织： iPSCs

I used at 3uM to generate cartilage from iPSCs, worked really well. Keep at -80C for long term storage

review image
Kidney organoids.
By Kirsty Rooney on 11/21/2020
分析类型： In Vitro
种属： Human
细胞系/组织： MAN13 hESCs

I use 8uM of CHIR to differentiate hESCs to intermediate mesoderm and then to kidney organoids

review image
Differentiation of human pluripotent stem cells.
By Paul Humphreys on 11/20/2020
分析类型： In Vitro
种属： Human
细胞系/组织： hPSCs

Directed differentiation of human pluripotent stem cells (hPSCs) towards mesoderm lineages. Used at concentration of 3uM. Very effective in induction of mesoderm markers.

Perform titration against your cell line to optimise correct concentration.

review image
iPSC differentiation.
By Stephanie Hsiao on 10/13/2020
分析类型： In Vitro
种属： Human
细胞系/组织： iPSC

Differentiation of human iPSCs into hematopoietic progenitor cells.

iPSC culture medium supplemented with 4 uM of CHIR99021.

review image
It worked good.
By Anonymous on 07/04/2020
分析类型： In Vitro
种属： Mouse
细胞系/组织： iPSC

Reprogramming media comprises of 3 μM CHIR99021 and 0.5 μM A-83-01.

The cells were then maintained in RM 15 days with fresh media every other day

PMID: 29681516
review image
For maintenance of stem cells pluripotency.
By Anonymous on 06/25/2020
分析类型： In Vitro
种属： Mouse
细胞系/组织： Neural Stem Cells

For directed differentiation to neural, cells are treated with GSK-3 inhibitor, CHIR99021 to induce canonical WNT signaling. This product gave me reproducible results.

It's better to aliquot and keep it at -80°C for long term storage.

PMID: 27133794
review image
Works as intended..
By Anonymous on 04/04/2020
分析类型： In Vitro
种属： Rat
细胞系/组织： Sprague-Dawley rats primary neuronal

10 μM for 2 h

PMID: 31291571 参考文献
review image
Differentiation of human dopaminergic neurons from stem cells.
By Anonymous on 02/17/2020
分析类型： In Vitro
种属： Human
细胞系/组织： Stem Cells

Used this product routinely to differentiate dopaminergic neurons from human stem cells.

review image
The hiPSCs were maintained with Essential 8 Flex Medium with CHIR 99021.
By Anonymous on 02/08/2020
分析类型： In Vitro
种属： Human
细胞系/组织： ipsc

1.4 μM

PMID: 29939162
review image
All iPSC lines and R1 mouse ES cells were maintained on feeders in KO-DMEM (Invitrogen) with 3 mM CHIR99021, and 1 mM PD0325901..
By Anonymous on 12/04/2019
分析类型： In Vitro
种属： Mouse
细胞系/组织： ipsc

All iPSC lines and R1 mouse ES cells were maintained on feeders in KO-DMEM (Invitrogen) with 3 mM CHIR99021, and 1 mM PD0325901.

PMID: 29358044
review image
works great for Wnt activation.
By avinash waghray on 08/08/2019
分析类型： In Vitro
种属： Human

In Tissue culture for growing basal cells

PMID: 27320041
Check the maintenance of naive pluripotency in 2i.
By Megha PB on 07/18/2018
分析类型： In Vitro
种属： Mouse
细胞系/组织： E14TG2a

Culture mouse ES cells in 2i media and without 2i to assess differentiation. Only the cells in 2i are pluripotent while mES cells cultured in -2i for 48 hours exit naive pluripotency.

review image
reliable product.
By Pinyuan Tian on 07/05/2018
分析类型： In Vitro
种属： Human
细胞系/组织： pluripotent stem cells

For directed differentiation of pluripotent stem cells to kidney organoids, cells are treated with GSK-3 inhibitor, CHIR99021 to induce canonical WNT signaling. CHIR99021 concentration and differentiation time period are the key factors for this protocol. I found this product gave me reproducible results.

Although the datasheet says store at -20°C, it's better to aliquot and keep it at -80°C for long term storage.

review image

该领域的文献

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。

Stem Cell Research Product Guide

Stem Cell Research Product Guide

This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:

  • Self-renewal and Maintenance
  • Differentiation
  • Reprogramming
  • Organoid Generation
  • GMP and Ancillary Material Grade Products
Stem Cells Scientific Review

Stem Cells Scientific Review

Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.

Alzheimer's Disease Poster

Alzheimer's Disease Poster

Alzheimer's disease (AD) is a debilitating and progressive neurodegenerative disease and the most common cause of dementia, affecting approximately 30% of individuals aged over 85 years. This poster summarizes the cellular and molecular mechanisms of AD.

Stem Cells Poster

Stem Cells Poster

Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.

Pathways for CHIR 99021

Wnt Signaling Pathway

Wnt Signaling Pathway

The Wnt pathway is involved in cellular differentiation and proliferation in adult tissues and also during embryogenesis. Disturbances within the pathway may lead to the formation of tumors and promote metastasis.