X-Gal

Discontinued Product

5510 has been discontinued.

View all Biochemicals and Molecular Biology products.
说明: Used in cloning procedures with IPTG
化学名: 5-Bromo-4-chloro-1H-indoll-3-yl-β-D-galactopyranoside
纯度: ≥98% (HPLC)
说明书
引用文献
评论 (1)

生物活性 for X-Gal

Chromogenic substrate of β-Galactosidase that produces blue color. Used in combination with IPTG (Cat. No. 5509) for detection of β-Galactosidase activity in bacterial colonies.

技术数据 for X-Gal

分子量 408.63
公式 C14H15BrClNO6
储存 Store at -20°C
纯度 ≥98% (HPLC)
CAS Number 7240-90-6
PubChem ID 65181
InChI Key OPIFSICVWOWJMJ-AEOCFKNESA-N
Smiles ClC1=C(C(O[C@H]3[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O3)=CN2)C2=CC=C1Br

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

产品说明书 for X-Gal

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参考文献 for X-Gal

参考文献是支持产品生物活性的出版物。

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关键词: X-Gal, X-Gal supplier, chromogenic, substrate, beta-galactosidase, β-galactosidase, b-galactosidase, Biochemicals, and, Molecular, Biology, 5510, Tocris Bioscience

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X gal for Blue White Screening.
By Anonymous on 02/04/2019
种属: Human

Bacterial cells were plated with L-agar, Ampillicin and X-gal solution at two different concentrations (5 and 50 µl) and a negative control containing only sterile water was set up. The plates were allowed to grow for 48 hours and counted for white and blue colonies.

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