10-DEBC hydrochloride

Name: 10-DEBC hydrochloride
Brand: Tocris Bioscience
SKU: 2558
Rating: 5 (1 reviews)

Cat. No. 2558 6 Citations 1 Review Submit a Review Datasheet / COA / SDS

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Description: Selective Akt/PKB inhibitor

Alternative Names: Akt Inhibitor X

Chemical Name: 10-[4'-(N,N-Diethylamino)butyl]-2-chlorophenoxazine hydrochloride

Purity: ≥99% (HPLC)

Datasheet

Citations (6)

Reviews (1)

Pathways (1)

Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for 10-DEBC hydrochloride

10-DEBC hydrochloride is a selective inhibitor of Akt/PKB. Inhibits IGF-1-stimulated phosphorylation and activation of Akt (complete inhibition at 2.5 μM), suppressing downstream activation of mTOR, p70 S6 kinase and S6 ribosomal protein. Shows no activity at PDK1, SGK1 or PI 3-kinase. Inhibits cell growth (IC₅₀ ~ 2-6 μM) and induces apoptosis in rhabdomyosarcoma cells.

Compound Libraries for 10-DEBC hydrochloride

10-DEBC hydrochloride is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library and Tocriscreen Antiviral Library. Find out more about compound libraries available from Tocris.

Technical Data for 10-DEBC hydrochloride

M. Wt	381.34
Formula	C₂₀H₂₅N₂OCl.HCl
Storage	Desiccate at +4°C
Purity	≥99% (HPLC)
CAS Number	925681-41-0
PubChem ID	16760284
InChI Key	SVKSJUIYYCQZEC-UHFFFAOYSA-N
Smiles	Cl.CCN(CC)CCCCN1C2=CC=CC=C2OC2=C1C=C(Cl)C=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for 10-DEBC hydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	38.13	100
	DMSO	38.13	100

Preparing Stock Solutions for 10-DEBC hydrochloride

The following data is based on the product molecular weight 381.34. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.62 mL	13.11 mL	26.22 mL
5 mM	0.52 mL	2.62 mL	5.24 mL
10 mM	0.26 mL	1.31 mL	2.62 mL
50 mM	0.05 mL	0.26 mL	0.52 mL

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Product Datasheets for 10-DEBC hydrochloride

Certificate of Analysis / Product Datasheet

Safety Datasheet

References for 10-DEBC hydrochloride

References are publications that support the biological activity of the product.

Thimmaiah et al (1992) Synthesis and chemical characterization of N-substituted phenoxazines directed toward reversing vinca alkaloid resistance in multidrug-resistant cancer cells. J.Med.Chem. 35 3358 PMID: 1527786

Thimmaiah et al (2005) Identification of N¹⁰-substituted phenoxazines as potent and specific inhibitors of Akt signaling. J.Biol.Chem. 36 31924

If you know of a relevant reference for 10-DEBC hydrochloride, please let us know.

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Keywords: 10-DEBC hydrochloride, 10-DEBC hydrochloride supplier, Selective, Akt, PKB, inhibitors, inhibits, Protein, Kinase, B, Kinases, Inhibitor, X, (Protein, B), B/Akt, 2558, Tocris Bioscience

6 Citations for 10-DEBC hydrochloride

Citations are publications that use Tocris products. Selected citations for 10-DEBC hydrochloride include:

Liddell et al (2016) Pyrrolidine dithiocarbamate activates the Nrf2 pathway in astrocytes. J Neuroinflammation 13 49 PMID: 26920699

Ali et al (2017) Isolation and characterization of a new naturally immortalized human breast carcinoma cell line, KAIMRC1. BMC Cancer 17 803 PMID: 29187162

Sokołowska et al (2014) Orexins protect neuronal cell cultures against hypoxic stress: an involvement of Akt signaling. PLoS One 52 48 PMID: 24243084

Waltero et al (2019) TOR as a Regulatory Target in Rhipicephalus microplus Embryogenesis. Front Physiol 10 965 PMID: 31417424

Gangoiti et al (2012) Ceramide 1-phosphate stimulates proliferation of C2C12 myoblasts. Biochimie 94 597 PMID: 21945811

Goodnough et al (2012) Inhibition of hepcidin transcription by growth factors. Hepatology 56 291 PMID: 22278715

Do you know of a great paper that uses 10-DEBC hydrochloride from Tocris? Please let us know.

Reviews for 10-DEBC hydrochloride

Average Rating: 5 (Based on 1 Review.)

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Inhibition of Akt/PKB in BV2 microglia cells.

By Joanna Plastira on 06/08/2019

Assay Type: In Vitro

Species: Mouse

Cell Line/Tissue: BV2 microglia cells

As part of our current study, we investigated the role of AKT pathway on the LPA induced inflammatory phenotype in microglia (downstream signaling). BV2 cells were incubated for different time points with LPA (1µM) in the presence or absence of AKT X (5µM). AKT inhibition totally decreased the expression of 2 pro-inflammatory transcription factors (pSTAT1 and pp65). The inhibitor was diluted in water.

Pathways for 10-DEBC hydrochloride

Akt Signaling Pathway

The Akt signaling pathway plays a key role in the mediation of protein synthesis, metabolism, proliferation and cell cycle progression. It may be referred to as a 'prosurvival' pathway.