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BCI

Cat. No. 7481 Submit a Review Datasheet / COA / SDS
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Description: DUSP6 and DUSP1 inhibitor
Chemical Name: 3-(Cyclohexylamino)-2,3-dihydro-2-(phenylmethylene)-1H-inden-1-one
Purity: ≥98% (HPLC)
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Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for BCI

BCI is an allosteric inhibitor of dual specificity phosphatase DUSP6 and DUSP1 (EC50 values are 8.0 μM and 13.3 μM respectively). Increases P2X7 receptor expression in neuroblastoma cells, reduces cell viability of EGFR or KRAS mutant lung cancer cells, and induces apoptosis in p53-null non-small cell lung cancer cells. In an animal model of myocardial infarction, BCI improves heart function, reduces fibrosis, and reduces inflammation by inhibiting macrophage formation. Activates FGF signaling in zebrafish embryo development to increase myocardial progenitor population and heart size.

Technical Data for BCI

M. Wt 317.42
Formula C22H23NO
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 15982-84-0
PubChem ID 6419844
InChI Key XJDKPLZUXCIMIS-HMMYKYKNSA-N
Smiles O=C1/C(C(C2=C1C=CC=C2)NC3CCCCC3)=C/C4=CC=CC=C4

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for BCI

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 31.74 100
ethanol 15.87 50

Preparing Stock Solutions for BCI

The following data is based on the product molecular weight 317.42. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.15 mL 15.75 mL 31.5 mL
5 mM 0.63 mL 3.15 mL 6.3 mL
10 mM 0.32 mL 1.58 mL 3.15 mL
50 mM 0.06 mL 0.32 mL 0.63 mL

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References for BCI

References are publications that support the biological activity of the product.

Zhang et al (2023) A DUSP6 inhibitor suppresses inflammatory cardiac remodeling and improves heart function after myocardial infarction. Dis.Model.Mech. 16 dmm049662 PMID: 36478044

Shin et al (2018) BCI induces apoptosis via generation of reactive oxygen species and activation of intrinsic mitochondrial pathway in H1299 lung cancer cells. Sci.China Life Sci. 61 1243 PMID: 29524123

Benito-León et al (2022) BCI, an inhibitor of the DUSP1 and DUSP6 dual specificity phosphatases, enhances P2X7 receptor expression in neuroblastoma cells. Front.Cell Dev.Biol. 15 1049566 PMID: 36589747

Unni et al (2018) Hyperactivation of ERK by multiple mechanisms is toxic to RTK-RAS mutation-driven lung adenocarcinoma cells. eLife 7 e33718 PMID: 30475204

If you know of a relevant reference for BCI, please let us know.

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Citations for BCI

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