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Description: PDE inhibitor (non-selective)
Chemical Name: 3,7-Dihydro-1-methyl-3-(2-methylpropyl)-1H-purine-2,6-dione
Purity: ≥98% (HPLC)
Biological Activity for IBMX
IBMX is a phosphodiesterase inhibitor (IC50 values are 13, 18, 19, 32 and 50 μM for PDE4, PDE3, PDE1, PDE5 and PDE2 respectively). Suppresses α-adrenoceptor-mediated 5-HT release from neuroendocrine epithelial cells (IC50 = 1.3 μM). Facilitates differentiation of neural progenitor cells in vitro.
Compound Libraries for IBMX
IBMX is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.
Technical Data for IBMX
| M. Wt | 222.24 |
| Formula | C10H14N4O2 |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 28822-58-4 |
| PubChem ID | 3758 |
| InChI Key | APIXJSLKIYYUKG-UHFFFAOYSA-N |
| Smiles | O=C1C2=C(N=CN2)N(CC(C)C)C(N1C)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for IBMX
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 16.67 | 75 |
Preparing Stock Solutions for IBMX
The following data is based on the product molecular weight 222.24. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.75 mM | 6 mL | 30 mL | 60 mL |
| 3.75 mM | 1.2 mL | 6 mL | 12 mL |
| 7.5 mM | 0.6 mL | 3 mL | 6 mL |
| 37.5 mM | 0.12 mL | 0.6 mL | 1.2 mL |
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Product Datasheets for IBMX
References for IBMX
References are publications that support the biological activity of the product.
Freitag et al (1998) Phosphodiesterase inhibitors suppress α2-adrenoceptor-mediated 5-hydroxytryptamine release from tracheae of newborn rabbits. Eur.J.Pharmacol. 354 67 PMID: 9726632
Lepski et al (2013) cAMP promotes the differentiation of neural progenitor cells in vitro via modulation of voltage-gated calcium channels. Front.Cell Neurosci. 7 155 PMID: 24065885
If you know of a relevant reference for IBMX, please let us know.
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Keywords: IBMX, IBMX supplier, PDE, inhibitors, inhibits, non-selective, Phosphodiesterases, Neural, Stem, Cells, 2845, Tocris Bioscience
18 Citations for IBMX
Citations are publications that use Tocris products. Selected citations for IBMX include:
Lundegaard et al (2015) MEK Inhibitors Reverse cAMP-Mediated Anxiety in Zebrafish. Chem Biol 22 1335 PMID: 26388333
Cuppoletti et al (2013) Methadone but not mor. inhibits lubiprostone-stimulated Cl- currents in T84 intestinal cells and recombinant human ClC-2, but not CFTR Cl- currents. Cell Biochem Biophys 66 53 PMID: 22918821
Jewell et al (2019) GPCR signaling inhibits mTORC1 via PKA phosphorylation of Raptor. Elife 8 PMID: 31112131
An et al (2019) Stem cell-derived cranial and spinal motor neurons reveal proteostatic differences between ALS resistant and sensitive motor neurons. Elife 8 PMID: 31157617
Burdyga et al (2018) Phosphatases control PKA-dependent functional microdomains at the outer mitochondrial membrane. Proc Natl Acad Sci U S A. 115 E6497 PMID: 29941564
Havekes et al (2016) Compartmentalized PDE4A5 Signaling Impairs Hippocampal Synaptic Plasticity and Long-Term Memory. J Neurosci 36 8936 PMID: 27559174
Cuppoletti et al (2014) Differentiation between human ClC-2 and CFTR Cl- channels with pharmacological agents. Am J Physiol Cell Physiol 307 C479 PMID: 25009109
Hook et al (2012) DArgic modulation of ganglion-cell photoreceptors in rat. Br J Pharmacol 35 507 PMID: 22304466
Cooke et al (2015) Morphine-induced internalization of the L83I mutant of the rat μ-opioid receptor. Biomed Res Int 172 593 PMID: 24697554
Ghosh et al (2015) The interplay between cyclic AMP, MAPK, and NF-κB pathways in response to proinflammatory signals in microglia. Eneuro 2015 308461 PMID: 25722974
Polito et al (2015) Selective Effects of PDE10A Inhibitors on Striatopallidal Neurons Require Phosphatase Inhibition by DARPP-32(1,2,3). PLoS One 2 PMID: 26465004
Merino et al (2015) Glucagon Increases Beating Rate but Not Contractility in Rat Right Atrium. Comparison with isoprote. Am J Physiol Lung Cell Mol Physiol 10 e0132884 PMID: 26222156
Patra et al (2018) Mutant GNAS drives pancreatic tumourigenesis by inducing PKA-mediated SIK suppression and reprogramming lipid metabolism. Nat Cell Biol 20 811 PMID: 29941929
Demeautis et al (2017) Multiplexing PKA and ERK12 kinases FRET biosensors in living cells using single excitation wavelength dual colour FLIM. Sci Rep 7 41026 PMID: 28106114
Hernández-Cascales (2017) Resveratrol enhances the inotropic effect but inhibits the proarrhythmic effect of sympathomimetic agents in rat myocardium. PeerJ 5 e3113 PMID: 28382232
Elliott et al (2015) Somatostatin and Ins mediate glucose-inhibited glucagon secretion in the pancreatic α-cell by lowering cAMP. Am J Physiol Endocrinol Metab 308 E130 PMID: 25406263
Jazayeri (2017) Crystal structure of the GLP-1 receptor bound to a peptide agonist. Nature 546 254 PMID: 28562585
Foster et al (2015) Low-glucose enhances keratocyte-characteristic phenotype from corneal stromal cells in serum-free conditions. Sci Rep 5 10839 PMID: 26039975
Do you know of a great paper that uses IBMX from Tocris? Please let us know.
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Rapid inhibition of PDE in acute brain slices.
By
Anonymous on 01/04/2018
Assay Type: Ex Vivo
Species: Mouse
Cell Line/Tissue: acute brain slice
Exogenous application of IBMX on brain slices
Effect is observed within minutes.