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Submit ReviewIonomycin calcium salt
Description: Calcium ionophore
Chemical Name: (4R,6S,8S,10Z,12R,14R,16E,18R,19R,20S,21S)-11,19,21-Trihydroxy-4,6,8,12,14,18,20-heptamethyl-22-[(2S,2'R,5S,5'S)-octahydro-5'-[(1R)-1-hydroxyethyl]-2,5'-dimethyl[2,2'-bifuran]-5-yl]-9-oxo-10,16-docosadienoic acid calcium salt
Purity: ≥97% (HPLC)
Biological Activity for Ionomycin calcium salt
Ionomycin calcium salt is a calcium ionophore; more specific than A23187.
Free Acid also available.
Technical Data for Ionomycin calcium salt
| M. Wt | 747.08 |
| Formula | C41H70O9.Ca |
| Storage | Store at -20°C |
| Purity | ≥97% (HPLC) |
| CAS Number | 56092-82-1 |
| PubChem ID | 6446270 |
| InChI Key | WKRWUYKLUMMAKG-WYGBAUISSA-L |
| Smiles | O=C([O-])CC[C@@H](C)C[C@H](C)C[C@H](C)C(/C=C([O-])/[C@H](C)C[C@@H](C/C=C/[C@@H](C)[C@@H](O)[C@@H](C)[C@@H](O)C[C@@H]1CC[C@]([C@]2([H])O[C@@]([C@H](O)C)(C)CC2)(C)O1)C)=O.[Ca+2] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Ionomycin calcium salt
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| ethanol | 74.71 | 100 | |
| DMSO | 18.68 | 25 with gentle warming |
Preparing Stock Solutions for Ionomycin calcium salt
The following data is based on the product molecular weight 747.08. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.34 mL | 6.69 mL | 13.39 mL |
| 5 mM | 0.27 mL | 1.34 mL | 2.68 mL |
| 10 mM | 0.13 mL | 0.67 mL | 1.34 mL |
| 50 mM | 0.03 mL | 0.13 mL | 0.27 mL |
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Product Datasheets for Ionomycin calcium salt
References for Ionomycin calcium salt
References are publications that support the biological activity of the product.
Elzi et al (2001) Ionomycin causes activation of p38 and p42/44 mitogen-activated protein kinases in human neutrophils. Am.J.Physiol.Cell Physiol. 281 C350 PMID: 11401859
Kaufmann et al (1980) Cation transport and specificity of ionomycin. Comparison with ionophore A23187 in rat liver mitochondria. J.Biol.Chem. 255 2735 PMID: 6766939
Liu and Hermann (1978) Characterization of ionomycin as a calcium ionophore. J.Biol.Chem. 253 5892 PMID: 28319
If you know of a relevant reference for Ionomycin calcium salt, please let us know.
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Keywords: Ionomycin calcium salt, Ionomycin calcium salt supplier, Calcium, ionophore, Ca2+, Ionophore, Signaling, Signalling, Agents, General, antibiotics, Ionophores, Antibiotics, 1704, Tocris Bioscience
17 Citations for Ionomycin calcium salt
Citations are publications that use Tocris products. Selected citations for Ionomycin calcium salt include:
Son et al (2015) Hypotonic stress induces RANKL via transient receptor potential melastatin 3 (TRPM3) and vaniloid 4 (TRPV4) in human PDL cells. J Dent Res 94 473 PMID: 25595364
Rojas et al (2013) Activation of group I metabotropic glutamate receptors potentiates heteromeric kainate receptors. Mol Pharmacol 83 106 PMID: 23066089
Shahidullah et al (2012) TRPV4 in porcine lens epithelium regulates hemichannel-mediated ATP release and Na-K-ATPase activity. Am J Physiol Cell Physiol 302 C1751 PMID: 22492652
Shambharkar et al (2015) TMEM203 Is a Novel Regulator of Intracellular Calcium Homeostasis and Is Required for Spermatogenesis. MBio 10 e0127480 PMID: 25996873
Azab et al (2015) Binding of αherpesvirus Glycoprotein H to Surface α4β1-Integrins Activates Calcium-Signaling Pathways and Induces Phosphatidylserine Exposure on the Plasma Membrane. PLoS One 6 PMID: 26489864
Jonas et al (2015) Assessment of TTX-s and TTX-r Action Potential Conduction along Neurites of NGF and GDNF Cultured Porcine DRG Somata. J Pharmacol Exp Ther 10 e0139107 PMID: 26407014
Selway et al (2012) Evidence that Ca2+ within the microdomain of the L-type voltage gated Ca2+ channel activates ERK in MIN6 cells in response to glucagon-like peptide-1. PLoS One 7 e33004 PMID: 22412973
Mikolajewicz et al (2018) Mechanically stimulated ATP release from murine bone cells is regulated by a balance of injury and repair. Elife 7 PMID: 30324907
Arcuri et al (2018) Probing Internalization Effects and Biocompatibility of Ultrasmall Zirconium Metal-Organic Frameworks UiO-66 NP in U251 Glioblastoma Cancer Cells. Nanomaterials (Basel) 8 PMID: 30360511
Mihara et al (2018) Involvement of VNUT-exocytosis in transient receptor potential vanilloid 4-dependent ATP release from gastrointestinal epithelium. PLoS One 13 e0206276 PMID: 30365528
Nieuwenhuijze et al (2018) Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell Alterations. Front Immunol 9 2234 PMID: 30323813
Plog et al (2015) Naturally Occurring Deletion Mutants of the Pig-Specific, Intestinal Crypt Epithelial Cell Protein CLCA4b without Apparent Phenotype. PLoS One 10 e0140050 PMID: 26474299
Makhortova et al (2011) A screen for regulators of survival of motor neuron protein levels. Nat Chem Biol 7 544 PMID: 21685895
Ohya et al (2011) Involvement of dominant-negative spliced variants of the intermediate conductance Ca2+-activated K+ channel, K(Ca)3.1, in immune function of lymphoid cells. J Biol Chem 286 16940 PMID: 21345794
Su et al (2019) Overexpression of P2X4 receptor in Schwann cells promotes motor and sensory functional recovery and remyelination via BDNF secretion after nerve injury. Glia 67 78 PMID: 30306657
Soltoff and Lannon (2013) Activation of ERK1/2 by store-operated calcium entry in rat parotid acinar cells. PLoS One 8 e72881 PMID: 24009711
Barcomb et al (2013) Enzymatic activity of CaMKII is not required for its interaction with the glutamate receptor subunit GluN2B. Mol Pharmacol 84 834 PMID: 24056996
Do you know of a great paper that uses Ionomycin calcium salt from Tocris? Please let us know.
Reviews for Ionomycin calcium salt
Average Rating: 5 (Based on 3 Reviews.)
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Good product for calcium imaging.
By
Yuyang Sun on 05/26/2022
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HSG
Used it for calcium image as positive control. Get significant trace increase after application.
Ionomycin Ca depletes intracellular Ca stores..
By
Anonymous on 01/11/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HEK293
HEK293 cells were loaded with intracellular Ca2+ sensitive dye, Fura-2AM in normal tyrode solution for 40 min in dark at room temperature, washed and kept in normal tyrode for 20 min. Fura2-loaded cells were excited at 340 and 380 nm and fluorescence at 510 nm was expressed as F340/F380 ratio, as an index of cytosolic Ca2+. Ca2+ stores were depleted with 1 µM ionomycin Ca in nominally Ca2+ free-tyrode solution, resulting in cytosolic Ca2+ peak.
Calcium increase.
By
Anonymous on 10/02/2018
Assay Type: In Vivo
Species: Mouse
Elicits calcium increase, used as a positive control at the end of calcium imaging experiments.