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Description: Fluorescent potassium ion (K+) indicator, membrane permeable
Chemical Name: Acetyloxymethyl 3-[3-(acetyloxymethoxy)-7-[3-(acetyloxymethoxy)-3-oxopropyl]-4,5-dichloro-9-[4-(5,17-dimethyl-8,14,24,27,32,35-hexaoxa-1,11,21-triazatetracyclo[19.8.8.02,7.015,20]heptatriaconta-2(7),3,5,15(20),16,18-hexaen-11-yl)-3-(2-methoxyethoxy)phenyl]-6-oxoxanthen-2-yl]propanoate
Purity: ≥90% (HPLC)
Biological Activity for IPG-4 AM
IPG-4 AM is a membrane-permeable fluorescent potassium ion (K+) indicator. It binds to potassium (Kd = 7 mM). Excitation and emission maxima (λ) are 525 and 545 nm, respectively. It has 100:1 selectivity for K+ over Na+ ions. It can be used to detect in real time changes in intracellular K+. Also used to monitor extracellular K+ changes in vivo.
It is recommended to prepare stock solutions in DMSO.
Optical Data for IPG-4 AM
| λabs | 525 nm |
|---|---|
| λem | 545 nm |
| Cell Permeable | Yes |
| Application | Confocal microscopy, Flow cytometry |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for IPG-4 AM
| M. Wt | 1333.27 |
| Formula | C67H79Cl2N3O21 |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 3019514-72-5 |
| PubChem ID | 163342041 |
| InChI Key | ORCBRKCLNTYBFH-UHFFFAOYSA-N |
| Smiles | O=C(C)OCOC(CCC1=CC2=C(C3=CC(OCCOC)=C(N(CCOC4=C5C=CC(C)=C4)CCOC6=C(N7CCOCCOCCN5CCOCCOCC7)C=CC(C)=C6)C=C3)C8=C(C(Cl)=C(C(CCC(OCOC(C)=O)=O)=C8)OCOC(C)=O)OC2=C(C1=O)Cl)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for IPG-4 AM
References for IPG-4 AM
References are publications that support the biological activity of the product.
Rana et al (2019) Calibration and characterization of intracellular Asante Potassium Green probes, APG-2 and APG-4. Anal.Biochem. 567 8 PMID: 30503709
Kikuchi et al (2022) Electrochemical potential enables dormant spores to integrate environmental signals. Science 378 43 PMID: 36201591
Humphries et al (2017) Species-independent attraction to biofilms through electrical signaling. Cell 168 200 PMID: 28086091
Hanalioglu et al (2022) Cortical spreading depression can be triggered by sensory stimulation in primed wild type mouse brain: a mechanistic insight to migraine aura generation. J.Headache Pain 23 107 PMID: 35986251
If you know of a relevant reference for IPG-4 AM, please let us know.
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Fluorescent Dyes and Probes Research Product Guide
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