Home / Product Type / Fluorescence Imaging / Fluorescent Probes and Stains / Fluorescent Ion Indicators / OG 488 BAPTA-1 AM

OG 488 BAPTA-1 AM

Cat. No. 6256 Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: Cell-permeable, fluorescent Ca2+ indicator
Chemical Name: N-[2-[(Acetyloxy)methoxy]-2-oxoethyl]-N-[4-[[[3',6'-bis(acetyloxy)-2',7'-difluoro-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl]carbonyl]amino]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]phenoxy]ethoxy]phenyl]glycine (acetyloxy)methyl ester
Purity: ≥90% (HPLC)
Datasheet
Citations
Reviews
Literature (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for OG 488 BAPTA-1 AM

OG 488 BAPTA-1 AM is a cell-permeable, fluorescently labeled Ca2+ indicator (Kd =170 nM). Composed of a green fluorescent probe (OG 488, Cat. No. 6257) conjugated to a cell-permeable Ca2+ chelator (BAPTA AM, Cat. No. 2787). Fluorescent intensity increases approximately 14-fold from resting intensity, upon Ca2+ binding. Displays uniform cellular distribution with minimal subcellular compartmentalization. Excitation/emission λ 494/523 nm.

Optical Data for OG 488 BAPTA-1 AM

Emission Color Green
λabs 494 nm
λem 523 nm
Extinction Coefficient (ε) 19000 M-1cm-1
Quantum Yield (φ) 0.91
Closest Laser line 488 nm
Cell Permeable Yes
Application Flow Cytometry, Fluorescence Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for OG 488 BAPTA-1 AM

M. Wt 1258.06
Formula C59H53F2N3O26
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 244167-57-5
PubChem ID 102028682
InChI Key NQVWLDWXFNDFLA-UHFFFAOYSA-N
Smiles FC1=CC2=C(C3=CC=C(C(NC4=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC5=CC=CC=C5N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C4)=O)C=C3C([O-])=O)C6=CC(F)=C(OC(C)=O)C=C6[O+]=C2C=C1OC(C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for OG 488 BAPTA-1 AM

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 1.26 1

Preparing Stock Solutions for OG 488 BAPTA-1 AM

The following data is based on the product molecular weight 1258.06. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.01 mM 79.49 mL 397.44 mL 794.87 mL
0.05 mM 15.9 mL 79.49 mL 158.97 mL
0.1 mM 7.95 mL 39.74 mL 79.49 mL
0.5 mM 1.59 mL 7.95 mL 15.9 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets for OG 488 BAPTA-1 AM

Certificate of Analysis / Product Datasheet
Select another batch:
Safety Datasheet

References for OG 488 BAPTA-1 AM

References are publications that support the biological activity of the product.

Thomas et al (2000) A comparison of fluorescent Ca2+ indicator properties and their use in measuring elementary and global Ca2+ signals Cell Calcium 28 213 PMID: 11032777

If you know of a relevant reference for OG 488 BAPTA-1 AM, please let us know.

View Related Products by Target

Keywords: OG 488 BAPTA-1 AM, OG 488 BAPTA-1 AM supplier, OG488, BAPTA-1AM, fluorescent, calcium, indicator, chelator, cell, permeable, Ion, Indicators, General, Calcium, Signaling, Agents, Fluorescent, 6256, Tocris Bioscience

Citations for OG 488 BAPTA-1 AM

Citations are publications that use Tocris products.

Currently there are no citations for OG 488 BAPTA-1 AM. Do you know of a great paper that uses OG 488 BAPTA-1 AM from Tocris? Please let us know.

Reviews for OG 488 BAPTA-1 AM

There are currently no reviews for this product. Be the first to review OG 488 BAPTA-1 AM and earn rewards!

Have you used OG 488 BAPTA-1 AM?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals