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Submit ReviewRyanodine
Description: Ca2+ release inhibitor
Chemical Name: 1H-Pyrrole-2-carboxylic acid, (3S,4R,4aR,6S,7S,8R,8aS,8bR,9S,9aS)-dodecahydro-4,6,7,8a,8b,9a-hexahydroxy-3,6a,9-trimethyl-7-(1-methylethyl)-6,9-methanobenzo[1,2]pentaleno[1,6-bc]furan-8-yl ester
Purity: ≥98% (HPLC)
Biological Activity for Ryanodine
Ryanodine is a potent inhibitor of Ca2+ release from sarcoplasmic reticulum (IC50 values are 10 and 2.8 nM in skeletal and cardiac muscle respectively).
Technical Data for Ryanodine
| M. Wt | 493.55 |
| Formula | C25H35NO9 |
| Storage | Desiccate at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 15662-33-6 |
| PubChem ID | 11317883 |
| InChI Key | JJSYXNQGLHBRRK-SFEDZAPPSA-N |
| Smiles | O=C(C6=CC=CN6)O[C@@H]1[C@@]([C@@H](C)C)(O)[C@]4(C)[C@@]3(O)[C@@](O)1[C@]5(C)[C@@]2(O)CC[C@H](C)[C@@H](O)[C@@]23O[C@](O)4C5 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Ryanodine
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| ethanol | 4.94 | 10 | |
| DMSO | 12.34 | 25 |
Preparing Stock Solutions for Ryanodine
The following data is based on the product molecular weight 493.55. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.25 mM | 8.1 mL | 40.52 mL | 81.05 mL |
| 1.25 mM | 1.62 mL | 8.1 mL | 16.21 mL |
| 2.5 mM | 0.81 mL | 4.05 mL | 8.1 mL |
| 12.5 mM | 0.16 mL | 0.81 mL | 1.62 mL |
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Product Datasheets for Ryanodine
References for Ryanodine
References are publications that support the biological activity of the product.
Bianchi (1997) Conformation state of the ryanodine receptor and functional effects of ryanodine on skeletal muscle. Biochem.Pharmacol. 53 909 PMID: 9174102
Sutko et al (1985) Ryanodine: a modifier of sarcoplasmic reticulum calcium release in striated muscle. Fed.Proc. 44 2984 PMID: 2415406
Sutko et al (1997) The pharmacology of ryanodine and related compounds. Pharmacol.Rev. 49 53 PMID: 9085309
If you know of a relevant reference for Ryanodine, please let us know.
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Keywords: Ryanodine, Ryanodine supplier, Ca2+, release, inhibitors, inhibits, Calcium, Signaling, Signalling, Agents, General, Ryanodine, Receptors, 1329, Tocris Bioscience
34 Citations for Ryanodine
Citations are publications that use Tocris products. Selected citations for Ryanodine include:
Li (2017) Metaplasticity mechanisms restore plasticity and associativity in an animal model of Alzheimer's disease. Proc Natl Acad Sci U.S.A. 114 5527 PMID: 28484012
Zhu et al (2015) Intracellular Ca(2+) release from endoplasmic reticulum regulates slow wave currents and pacemaker activity of interstitial cells of Cajal. Am J Physiol Cell Physiol 308 C608 PMID: 25631870
Gruol et al (2012) Ethanol alters opioid regulation of Ca(2+) influx through L-type Ca(2+) channels in PC12 cells. Alcohol Clin Exp Res 36 443 PMID: 22014285
Luciani et al (2009) Roles of IP3R and RyR Ca2+ channels in endoplasmic reticulum stress and beta-cell death. Diabetes 58 422 PMID: 19033399
Tyser et al (2016) Calcium handling precedes cardiac differentiation to initiate the first heartbeat. Elife 5 PMID: 27725084
Naka et al (2013) Non-Hebbian plasticity at C-fiber synapses in rat spinal cord lamina I neurons. Pain 154 1333 PMID: 23707311
Huang and Hsu (2010) Activation of muscarinic acetylcholine receptors induces a nitric oxide-dependent long-term depression in rat medial prefrontal cortex. Cereb Cortex 20 982 PMID: 19666830
Dera et al (2014) Modified cytoplasmic Ca2+ sequestration contributes to spinal cord injury-induced augmentation of nerve-evoked contractions in the rat tail artery. PLoS One 9 e111804 PMID: 25350563
Zhou et al (2012) Local small airway epithelial injury induces global smooth muscle contraction and airway constriction. J Appl Physiol (1985) 112 627 PMID: 22114176
Few et al (2012) Asynchronous Ca2+ current conducted by voltage-gated Ca2+ (CaV)-2.1 and CaV2.2 channels and its implications for asynchronous neurotransmitter release. Proc Natl Acad Sci U S A 109 E452 PMID: 22308469
Begum et al (2012) DHA inhibits ER Ca2+ release and ER stress in astrocytes following in vitro ischemia. J Neurochem 120 622 PMID: 22129278
Low et al (2012) Netrin-1 stimulates developing GnRH neurons to extend neurites to the median eminence in a calcium- dependent manner. PLoS One 7 e46999 PMID: 23056554
Karwatsky et al (2010) Cholesterol efflux to apoA-I in ABCA1-expressing cells is regulated by Ca2+-dependent calcineurin signaling. J Lipid Res 51 1144 PMID: 19965585
Jourdain et al (2018) Dual action of L-Lactate on the activity of NR2B-containing NMDA receptors: from potentiation to neuroprotection. Sci Rep 8 13472 PMID: 30194439
Zhang et al (2016) Signaling mechanism for modulation by ATP of glycine receptors on rat retinal ganglion cells. Sci Rep 6 28938 PMID: 27357477
Zhang et al (2015) Cardiac sodium/calcium exchanger preconditioning promotes anti-arrhythmic and cardioprotective effects through mitochondrial calcium-activated potassium channel. Pflugers Arch 8 10239 PMID: 26617732
Dobson et al (2015) Caffeine Modulates Vesicle Release and Recovery at Cerebellar Parallel Fibre Terminals, Independently of Calcium and Cyclic AMP Signalling. J Neurosci Res 10 e0125974 PMID: 25933382
Nordman and Kabbani (2014) Microtubule dynamics at the growth cone are mediated by α7 nicotinic receptor activation of a Gαq and IP3 receptor pathway. J Neurosci 28 2995 PMID: 24687992
Kim et al (2010) Non-cardiomyocytes influence the electrophysiological maturation of human embryonic stem cell-derived cardiomyocytes during differentiation. Int J Clin Exp Pathol 19 783 PMID: 20001453
Xu et al (2011) The human immunodeficiency virus coat protein gp120 promotes forward trafficking and surface clustering of NMDA receptors in membrane microdomains. PLoS One 31 17074 PMID: 22114277
Kim et al (2011) Alteration of Ryanodine-receptors in Cultured Rat Aortic Smooth Muscle Cells. PLoS One 15 431 PMID: 22359482
Babola et al (2020) Purinergic signaling in cochlear supporting cells reduces hair cell excitability by increasing the extracellular space. ELife 9 e52160 PMID: 31913121
Seto et al (2013) Acute SimV. inhibits K ATP channels of porcine coronary artery myocytes. Am J Physiol Cell Physiol 8 e66404 PMID: 23799098
Kyle et al (2013) Mechanisms underlying activation of transient BK current in rabbit urethral smooth muscle cells and its modulation by IP3-generating agonists. Korean J Physiol Pharmacol 305 C609 PMID: 23804200
Lotteau et al (2013) Characterization of functional TRPV1 channels in the sarcoplasmic reticulum of mouse skeletal muscle. PLoS One 8 e58673 PMID: 23536811
Fernández-Velasco et al (2011) RyRCa2+ leak limits cardiac Ca2+ window current overcoming the tonic effect of calmodulinin mice. PLoS One 6 e20863 PMID: 21673970
Li and Burrell (2011) Associative, bidirectional changes in neural signaling utilizing NMDA receptor- and endocannabinoid-dependent mechanisms. Learn Mem 18 545 PMID: 21844187
Florea et al (2017) Calcium-regulatory proteins as modulators of chemotherapy in human neuroblastoma. Oncotarget 8 22876 PMID: 28206967
Gerich et al (2009) H(2)O(2)-mediated modulation of cytosolic signaling and organelle function in rat hippocampus. FASEB J 458 937 PMID: 19430810
Dror et al (2008) Glucose and endoplasmic reticulum calcium channels regulate HIF-1beta via presenilin in pancreatic beta-cells. J Biol Chem 283 9909 PMID: 18174159
Henrich and Buckler (2008) Effects of anoxia and aglycemia on cytosolic calcium regulation in rat sensory neurons. J Neurophysiol 100 456 PMID: 18417627
Larina and Thorn (2005) Ca2+ dynamics in salivary acinar cells: distinct morphology of the acinar lumen underlies near-synchronous global Ca2+ responses. J Cell Sci 118 4131 PMID: 16118245
Dai et al (2006) Apelin increases contractility in failing cardiac muscle. Eur J Pharmacol 553 222 PMID: 17055480
Gascon et al (2006) GABA regulates dendritic growth by stabilizing lamellipodia in newly generated interneurons of the olfactory bulb. J Neurosci 26 12956 PMID: 17167085
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