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Description: Potent BMP4 agonist
Chemical Name: 2-[[(4-Bromophenyl)methyl]thio]benzoxazole
Purity: ≥99% (HPLC)
Biological Activity for SB 4
SB 4 is a potent BMP4 agonist (EC50 = 74 nM). Activates canonical BMP signaling and increases SMAD-1/5/9 phosphorylation, with no effect on p-SMAD-2, p-SMAD-3 or p-TAK1. Bypasses noggin-induced inhibition of type I BMP receptors. When used in combination, SB 4 enhances BMP4 signaling and embryonic stem cell differentiation.
Compound Libraries for SB 4
SB 4 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.
Technical Data for SB 4
| M. Wt | 320.2 |
| Formula | C14H10BrNOS |
| Storage | Store at -20°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 100874-08-6 |
| PubChem ID | 731364 |
| InChI Key | JDVOIBFEFVKUPL-UHFFFAOYSA-N |
| Smiles | BrC1=CC=C(C=C1)CSC2=NC3=C(C=CC=C3)O2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for SB 4
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 32.02 | 100 | |
| ethanol | 6.4 | 20 |
Preparing Stock Solutions for SB 4
The following data is based on the product molecular weight 320.2. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.12 mL | 15.62 mL | 31.23 mL |
| 5 mM | 0.62 mL | 3.12 mL | 6.25 mL |
| 10 mM | 0.31 mL | 1.56 mL | 3.12 mL |
| 50 mM | 0.06 mL | 0.31 mL | 0.62 mL |
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Product Datasheets for SB 4
References for SB 4
References are publications that support the biological activity of the product.
Bradford et al (2019) High-throughput screens for agonists of bone morphogenetic protein (BMP) signaling identify potent benzoxazole compounds. J.Biol.Chem. 294 3125 PMID: 30602563
Hayaei Tehrani et al (2022) A comparison between BMP4 and SB4 in inducing germ line gene expression pattern during embryonic stem cells differentiation. Differentiation 123 9 PMID: 34864442
If you know of a relevant reference for SB 4, please let us know.
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Keywords: SB 4, SB 4 supplier, SB4, Potent, BMP4, agonists, agonism, SMAD, phosphorylation, canonical, BMP, signaling, activators, stem, cell, differentiation, and, Other, Activin, Receptors, ESCs, iPSC, 6881, Tocris Bioscience
1 Citation for SB 4
Citations are publications that use Tocris products. Selected citations for SB 4 include:
Hayaei Tehrani et al (2022) A comparison between BMP4 and SB4 in inducing germ line gene expression pattern during embryonic stem cells differentiation. Differentiation 123 9 PMID: 34864442
Do you know of a great paper that uses SB 4 from Tocris? Please let us know.
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Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Stem Cell Research Product Guide
This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:
- Self-renewal and Maintenance
- Differentiation
- Reprogramming
- Organoid Generation
- GMP and Ancillary Material Grade Products
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
Stem Cell Workflow Poster
Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.
Stem Cells Poster
Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.
Pathways for SB 4
