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Description: Fluorogen for SiRA light-up aptamer. Application: confocal microscopy, SRM (including STED)
Chemical Name: (5-((2-(2-(2-Aminoethoxy)ethoxy)ethyl)carbamoyl)-2-(7-(dimethylamino)-3-(dimethyliminio)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate)
Purity: ≥98% (HPLC)
Biological Activity for SiRA 2
Key information: SiRA 2 (silicon rhodamine 2) is a near-infrared (NIR) fluorogen for SiRA light-up aptamer. SiRA 2 enables imaging of single RNA in living cells.
Application: confocal microscopy, super resolution microscopy (SRM) techniques, including super-resolution stimulated emission depletion (STED). Cell permeable.
Properties and Photophysical Data: SiRA 2 is extremely bright, photostable and allows the visualization of mRNA expression in bacteria in no-wash, quencher-free, live-cell imaging experiments. Excitation and emission maxima (λ) are 649 nM and 662 nM, respectively; quantum yield = 0.98; extinction coefficient = 86,000 M-1cm-1; KD = 403 nM.
Reconstitute in 200 μL for a 0.5 mM stock solution. A typical working concentration for this product is 500 nM, see references provided for more details.
We recommend using 5mM Mg2+ (MgCl2 or MgSO4) in all buffers/media for maximum fluorescence intensity.
Licensing Information
Sold with kind permission of Prof. Andres Jäschke
Optical Data for SiRA 2
| Emission Color | Far Red |
|---|---|
| λabs | 649 nm |
| λem | 662 nm |
| Extinction Coefficient (ε) | 86000 M-1cm-1 |
| Quantum Yield (φ) | 0.98 |
| Closest Laser line | 640 nm |
| Cell Permeable | Yes |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for SiRA 2
| M. Wt | 602.81 |
| Formula | C33H42N4O5Si |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 2365417-91-8 |
| PubChem ID | 168440271 |
| InChI Key | VZACMAUVTBSQSR-UHFFFAOYSA-N |
| Smiles | CN(C1=CC=C2C([Si](C)(C3=C/C(C=CC3=C2C4=C(C([O-])=O)C=C(C(NCCOCCOCCN)=O)C=C4)=[N+](C)\C)C)=C1)C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for SiRA 2
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 60.28 | 100 | |
| water | 30.14 | 50 |
Preparing Stock Solutions for SiRA 2
The following data is based on the product molecular weight 602.81. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.66 mL | 8.29 mL | 16.59 mL |
| 5 mM | 0.33 mL | 1.66 mL | 3.32 mL |
| 10 mM | 0.17 mL | 0.83 mL | 1.66 mL |
| 50 mM | 0.03 mL | 0.17 mL | 0.33 mL |
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Product Datasheets for SiRA 2
References for SiRA 2
References are publications that support the biological activity of the product.
Wirth et al (2019) SiRA: A silicon rhodamine-binding aptamer for live-cell super-resolution RNA imaging. J.Am.Chem.Soc. 141 7562 PMID: 30986047
If you know of a relevant reference for SiRA 2, please let us know.
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Keywords: SiRA 2, SiRA 2 supplier, SiRA2, fluorescence, fluorophores, single, RNA, imaging, fluorescent, aptamers, aptamer-based, silicon, rhodamine, NIR, near-infrared, live, cell, STED, SRM, Aptamer-based, Imaging, Fluorescent, Probes, 7544, Tocris Bioscience
Citations for SiRA 2
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals