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Submit ReviewSynaptoRedTM C2
Description: Fluorescent dye. Used for: synaptic vesicles staining. Application: fluorescence microscopy
Alternative Names: FM4-64
Chemical Name: 4-[6-[4-(Diethylamino)phenyl]-1,3,5-hexatrien-1-yl]-1-[3-(triethylammonio)propyl]pyridinium dibromide
Purity: ≥95% (HPLC)
Biological Activity for SynaptoRedTM C2
Key information: SynaptoRed™ C2 is a fluorescent dye for synaptic vesicles staining.
Used for: Suitable for monitoring synaptic activity at the synapse or neuromuscular junctions.
Application: fluorescence microscopy.
Properties and Photophysical Data: excitation and emission maxima (λ) are 510 nm and 750 nm, respectively.
Licensing Information
SynaptoRed is a trademark of Biotium, Inc.
Technical Data for SynaptoRedTM C2
| M. Wt | 607.51 |
| Formula | C30H45Br2N3 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 162112-35-8 |
| PubChem ID | 2733635 |
| InChI Key | AFVSZGYRRUMOFH-UHFFFAOYSA-L |
| Smiles | CCN(CC)C(C=C2)=CC=C2\C=C/C=C/C=C\C1=CC=[N+](CCC[N+](CC)(CC)CC)C=C1.[Br-].[Br-] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for SynaptoRedTM C2
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 10 | 17 |
Preparing Stock Solutions for SynaptoRedTM C2
The following data is based on the product molecular weight 607.51. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.17 mM | 9.68 mL | 48.41 mL | 96.83 mL |
| 0.85 mM | 1.94 mL | 9.68 mL | 19.37 mL |
| 1.7 mM | 0.97 mL | 4.84 mL | 9.68 mL |
| 8.5 mM | 0.19 mL | 0.97 mL | 1.94 mL |
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Product Datasheets for SynaptoRedTM C2
References for SynaptoRedTM C2
References are publications that support the biological activity of the product.
Daniel et al (2012) Analysis of synaptic vesicle endocytosis in synaptosomes by high-content screening. Nat.Protoc. 7 1439 PMID: 22767087
Fischer-Parton et al (2000) Confocal microscopy of FM4-64 as a tool for analysing endocytosis and vesicle trafficking in living fungal hyphae. J.Microsc. 198 246 PMID: 10849201
Dason et al (2010) Vesicular sterols are essential for synaptic vesicle cycling. J.Neurosci. 30 15856 PMID: 21106824
If you know of a relevant reference for SynaptoRedTM C2, please let us know.
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Keywords: SynaptoRed C2, SynaptoRed C2 supplier, Fluorescent, probe, FM4-64, excitation, wavelength, emission, synaptic, vesicles, Organelle, and, Cell, Structure, Stains, Histology, Neuron, Astrocyte, Probes, Lipid, Membrane, 5118, Tocris Bioscience
1 Citation for SynaptoRedTM C2
Citations are publications that use Tocris products. Selected citations for SynaptoRedTM C2 include:
Richard et al (2021) Plant cell adhesion and growth on artificial fibrous scaffolds as an in vitro model for plant development. Sci Adv 7 eabj1469 PMID: 34669469
Do you know of a great paper that uses SynaptoRedTM C2 from Tocris? Please let us know.
Reviews for SynaptoRedTM C2
Average Rating: 4 (Based on 2 Reviews.)
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Filter by:
synaptic vesicle dye.
By
Karthik Krishnamurthy on 08/03/2021
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: cortical neurons
Cultured cortical neurons labeled with 5 micromolar SynaptoRed for 5 minutes upon KCl depolarization and washing with aCSF.
synaptic vesicle release.
By
Anonymous on 12/12/2017
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: motor neurons
for synaptic vesicle recycling studies in cortical and motor neurons
10 micromolar of synaptored was used
Literature in this Area
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Fluorescent Dyes and Probes Research Product Guide
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