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Imaging Applications

Tocris offers an extensive range of fluorescent and bioluminescent dyes, probes, indicators, buffers and more to support all your fluorescent imaging needs. We have grouped all the main dyes, counter stains and buffers according to applications below. You will also find links to the comprehensive range of antibodies from our sister brands R&D Systems™ and Novus Biologicals, including over 40,000 IHC validated antibodies.

Targets
Background
Literature (1)

Spectra Viewer

Use our spectra viewer to interactively plan your imaging experiments. View the excitation and emission data for all common dyes including Janelia Fluor ® dyes, Cyanine Dyes (Cy Dyes), TFAX (AF) dyes and BDY (BODIPY®) dyes. Note: absorption spectra may be displayed instead of excitation spectra.

Use our spectra viewer to plan your flow cytometry experiments

Featured Product Range

Janelia Fluor® Dyes

Janelia Fluor® (JF) dyes are bright, cell permeable and highly photostable fluorescent dyes, which are especially well suited to live-cell imaging and super-resolution techniques, as well as traditional confocal microscopy. Janelia Fluor® dyes are compatible with self-labeling tag systems e.g. HaloTag® and SNAP-tag®. Janelia Fluor® dyes are supplied with several options for reactive groups including tetrazine, azide, free acid, maleimide, and NHS ester (SE). Biomolecule conjugation protocols available. Janelia Fluor® photoactivatable dyes are also available for PALM microscopy

Application of Janelia Fluor® Dyes in Cardiac Tissue

Experiments using Janelia Fluor® Dyes in Cardiac Tissue - Left - displaying collagen VI in the interstitial space between muscle cells. Labeled with a primary ab against collagen VI and a secondary ab conjugated to JF549 (#6147). Scale: 50 μm. Middle - adult pig heart tissue, 10 μm thick, labeled with abs against SERCA2ATPase, displays sarcoplasmic reticulum. Top: super-resolution image (dSTORM), bottom: diffraction limited widefield image. Scale: 200 nm. Right - Cardiomyocyte stained against α-actinin, displaying its periodic structure localised at the ends of sarcomeres (Z-discs). (left) Widefield fluorescence of JF549 (right) dSTORM super-resolution image (right). Scale: 1 μm.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister brand Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Literature for Imaging Applications

Tocris offers the following scientific literature for Imaging Applications to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals