Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit Review4-Thiouridine
Description: Photoreactive uridine analog for RNA sequencing
Chemical Name: 4-Thiouridine
Purity: ≥98% (HPLC)
Biological Activity for 4-Thiouridine
4-Thiouridine is a photoreactive uridine analog for RNA sequencing (RNA seq) and thiol(SH)-linked alkylation for the metabolic sequencing of RNA (SLAM seq). Used as a crosslinker to identify RNA binding proteins. Is incorporated into newly transcribed RNA and can be used to track RNA from synthesis to degradation.
Technical Data for 4-Thiouridine
| M. Wt | 260.26 |
| Formula | C9H12N2O5S |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 13957-31-8 |
| PubChem ID | 3032615 |
| InChI Key | ZLOIGESWDJYCTF-XVFCMESISA-N |
| Smiles | OC[C@H]1O[C@@H](N2C=CC(NC2=O)=S)[C@@H]([C@@H]1O)O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for 4-Thiouridine
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 26.03 | 100 | |
| DMSO | 26.03 | 100 |
Preparing Stock Solutions for 4-Thiouridine
The following data is based on the product molecular weight 260.26. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.84 mL | 19.21 mL | 38.42 mL |
| 5 mM | 0.77 mL | 3.84 mL | 7.68 mL |
| 10 mM | 0.38 mL | 1.92 mL | 3.84 mL |
| 50 mM | 0.08 mL | 0.38 mL | 0.77 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for 4-Thiouridine
References for 4-Thiouridine
References are publications that support the biological activity of the product.
Garibaldi et al (2017) Isolation of newly transcribed RNA using the metabolic label 4-thiouridine. Methods Mol.Biol. 1648 169 PMID: 28766297
Herzog et al (2017) Thiol-linked alkylation of RNA to assess expression dynamics. Nat.Methods 14 1198 PMID: 28945705
Schofield et al (2018) TimeLapse-seq: adding a temporal dimension to RNA sequencing through nucleoside recoding. Nat.Methods 15 221 PMID: 29355846
If you know of a relevant reference for 4-Thiouridine, please let us know.
View Related Products by Target
Keywords: 4-Thiouridine, 4-Thiouridine supplier, 4sU, tagging, photoreactive, labels, labelling, labeling, RNA, seq, sequencing, SLAM, TimeLapse, Biochemicals, and, Molecular, Biology, 7005, Tocris Bioscience
2 Citations for 4-Thiouridine
Citations are publications that use Tocris products. Selected citations for 4-Thiouridine include:
Cindy et al (2023) In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion. Nat Commun 14 3386 PMID: 37296170
Ali et al (2021) Genetic Deletion of Polo-Like Kinase 2 Induces a Pro-Fibrotic Pulmonary Phenotype. Cells 10 PMID: 33799608
Do you know of a great paper that uses 4-Thiouridine from Tocris? Please let us know.
Reviews for 4-Thiouridine
There are currently no reviews for this product. Be the first to review 4-Thiouridine and earn rewards!
Have you used 4-Thiouridine?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image