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Submit ReviewBAM (8-22)
Description: Potent MRGPRX1 agonist
Purity: ≥95% (HPLC)
Biological Activity for BAM (8-22)
BAM (8-22) is an endogenous peptide fragment that is a potent agonist for MRGPRX1 (EC50 values are 8 - 150 nM). It was first isolated from bovine adrenal medulla. Unlike BAM 22P (Cat. No. 1650), BAM (8-22) does not contain the met-enkephalin motif therefore displays no affinity for opioid receptors. BAM (8-22) augments scratching behavior in a cholestatic pruritus mice model. BAM (8-22) attenuates bone cancer pain in mice.
Technical Data for BAM (8-22)
| M. Wt | 1971.22 |
| Formula | C91H127N25O23S |
| Sequence | VGRPEWWMDYQKRYG |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 412961-36-5 |
| PubChem ID | 16158367 |
| InChI Key | GOEYECACIBFJGZ-NPAGUKBMSA-N |
| Smiles | [H]N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)NCC(O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for BAM (8-22)
| Solubility | Soluble to 1 mg/ml in water |
Product Datasheets for BAM (8-22)
References for BAM (8-22)
References are publications that support the biological activity of the product.
Chen and Ikeda (2004) Modulation of ion channels and synaptic transmission by a human sensory neuron-specific G-protein-coupled receptor, SNSR4/mrgX1, heterologously expressed in cultured rat neurons. J.Neurosci. 24 5044 PMID: 15163697
Lembo et al (2002) Proenkephalin A gene products activate a new family of sensory neuron-specific GPCRs. Nat.Neurosci. 5 201 PMID: 11850634
Simonin and Kieffer (2002) Two faces for an opioid peptide - and more receptors for pain research. Nat.Neurosci. 5 185 PMID: 11865303
Solinski et al (2014) Pharmacology and signaling of MAS-related G protein-coupled receptors. Pharmacol.Rev 66 570 PMID: 24867890
Sanjel et al (2019) BAM8-22 and its receptor MRGPRX1 may attribute to cholestatic pruritus. Sci.Rep. 9 10888 PMID: 31350433
Sun et al (2016) Activation of spinal MrgC-Gi-NR2B-nNOS signaling pathway by Mas oncogene-related gene C receptor agonist bovine adrenal medulla 8-22 attenuates bone cancer pain in mice. Am.J.Transl.Res. 8 1144 PMID: 27158400
If you know of a relevant reference for BAM (8-22), please let us know.
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Keywords: BAM (8-22), BAM (8-22) supplier, Selective, agonists, sensory, neuron-specific, receptors, SNSR, MRGPRX1, mas-related, g, protein, coupled, agonism, potent, Mas-related, G, Protein-Coupled, Receptors, 1763, Tocris Bioscience
5 Citations for BAM (8-22)
Citations are publications that use Tocris products. Selected citations for BAM (8-22) include:
Sanjel et al (2019) BAM8-22 and its receptor MRGPRX1 may attribute to cholestatic pruritus. Sci.Rep. 9 10888 PMID: 31350433
Honda et al (2019) Effects of intravesical administration of sensory neuron-specific receptor agonist on voiding function in rats with CP-induced cystitis. Cent European J Urol 72 142 PMID: 31482020
Hin et al (2014) Peptidomimetics of Arg-Phe-NH2 as small molecule agonists of Mas-related gene C (MrgC) receptors. Bioorg Med Chem 22 5831 PMID: 25288495
Grundy et al (2021) Activation of MrgprA3 and MrgprC11 on bladder-innervating afferents induces peripheral and central hypersensitivity to bladder distension. J.Neurosci. 41 3900 PMID: 33727332
Moser and Giesler (2013) Itch and analgesia resulting from intrathecal application of morphine: contrasting effects on different populations of trigeminothalamic tract neurons. J Neurosci 33 6093 PMID: 23554490
Do you know of a great paper that uses BAM (8-22) from Tocris? Please let us know.
Reviews for BAM (8-22)
Average Rating: 5 (Based on 1 Review.)
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Excellent In vivo and ex vivo activity with miminal lot variation.
By
Jeff Guo on 11/30/2023
Assay Type: Ex Vivo
Species: Mouse
Cell Line/Tissue: Dorsal Root Ganglia
Demonstrates excellent bio-activity and virtually free of batch/lot-effects in both in vivo mouse behavioral assays (low mM concentrations) and ex vivo calcium imaging assays (low μM concentrations). Highly recommended to minimize variation, for critical assays, and for generating publication quality data.Attached image shows representative calcium response of ex vivo dorsal root ganglia in response to bath applied 10 μM BAM 8-22. Image is composite of GCaMP3 fluorescence intensity over 180 seconds, after baseline subtraction, with contract and brightness adjustments to improve image clarity.
