HEPES

Cat. No. 3173 1 Citation 3 Reviews Submit a Review Datasheet / COA / SDS

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Description: Multipurpose HEPES buffer; used in cell culture

Chemical Name: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid

Purity: ≥99.5%

Datasheet

Citations (1)

Reviews (3)

Protocols (1)

Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for HEPES

HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.

For more information about how HEPES may be used, see our protocol:

3D Culture of Lung Alveolar Cells

Technical Data for HEPES

M. Wt	238.3
Formula	C₈H₁₈N₂O₄S
Storage	Store at RT
Purity	≥99.5%
CAS Number	7365-45-9
PubChem ID	23830
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Smiles	OCCN1CCN(CCS(=O)(O)=O)CC1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for HEPES

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	238.3	1000

Preparing Stock Solutions for HEPES

The following data is based on the product molecular weight 238.3. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
10 mM	0.42 mL	2.1 mL	4.2 mL
50 mM	0.08 mL	0.42 mL	0.84 mL
100 mM	0.04 mL	0.21 mL	0.42 mL
500 mM	0.01 mL	0.04 mL	0.08 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

Dilution Calculator

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Product Datasheets for HEPES

Certificate of Analysis / Product Datasheet

Safety Datasheet

References for HEPES

References are publications that support the biological activity of the product.

Good et al (1966) Hydrogen ion buffers for biological research. Biochemistry 5 467 PMID: 5942950

Medzon and Gedies (1971) Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells. Canadian J.Microbiol. 17 651

If you know of a relevant reference for HEPES, please let us know.

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Keywords: HEPES, HEPES supplier, Multipurpose, buffer, Reagents, cell, culture, ph, Buffers,, Solvents, and, Solutions, Organoids, 3173, Tocris Bioscience

1 Citation for HEPES

Citations are publications that use Tocris products. Selected citations for HEPES include:

Lerner et al (2010) Endocannabinoid signaling mediates psychomotor activation by adenosine A2A antagonists. J Neurosci 30 2160 PMID: 20147543

Do you know of a great paper that uses HEPES from Tocris? Please let us know.

Reviews for HEPES

Average Rating: 4.3 (Based on 3 Reviews.)

5 Star

33%

4 Star

67%

3 Star

2 Star

1 Star

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HEPES (10 mM) maintains pH in the experimental buffer for Ca2+ imaging..

By Anonymous on 07/02/2019

Assay Type: In Vitro

Species: Human

Cell Line/Tissue: HEK293

HEPES (10 mM) is used to maintain pH 7.4 in the experimental buffer for Ca2+ imaging, consisting of 137 mM NaCl, 5.4 mM KCl, 1 mM CaCl2, 1 mM MgCl2 and 10 mM glucose.

Good buffer.

By Anonymous on 08/05/2018

Assay Type: Ex Vivo

Species: Mouse

Used to buffer Krebs buffer for pressure myography. Good performance, maintained pH without any problem. I would use it again.

HEPES to buffer pH.

By Anonymous on 01/31/2018