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Description: Broad spectrum cysteine reactive probe
Chemical Name: N-5-Hexyn-1-yl-2-iodoacetamide
Purity: ≥97%
Biological Activity for IA-Alkyne
IA-Alkyne is a broad spectrum cysteine reactive probe. Covalently binds cysteine residues in proteins. Can be utilized for covalent fragment-based ligand discovery (FBLD) and to identify Zn2+-binding cysteines. Used as a click-chemistry handle to attach a fluorophore or biotin to reactive cysteines in proteins for visualization by in-gel fluorescence or identification by mass spectrometry, respectively. Covalently binds cysteine residues in and activates TRPA1 channels.
Technical Data for IA-Alkyne
| M. Wt | 265.09 |
| Formula | C8H12INO |
| Storage | Store at -20°C |
| Purity | ≥97% |
| CAS Number | 930800-38-7 |
| PubChem ID | 44448710 |
| InChI Key | YCRUVTMZPHEOAM-UHFFFAOYSA-N |
| Smiles | ICC(=O)NCCCCC#C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for IA-Alkyne
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 26.51 | 100 | |
| ethanol | 26.51 | 100 |
Preparing Stock Solutions for IA-Alkyne
The following data is based on the product molecular weight 265.09. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.77 mL | 18.86 mL | 37.72 mL |
| 5 mM | 0.75 mL | 3.77 mL | 7.54 mL |
| 10 mM | 0.38 mL | 1.89 mL | 3.77 mL |
| 50 mM | 0.08 mL | 0.38 mL | 0.75 mL |
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Product Datasheets for IA-Alkyne
References for IA-Alkyne
References are publications that support the biological activity of the product.
Backus et al (2016) Proteome-wide covalent ligand discovery in native biological systems. Nature 534 570 PMID: 27309814
Pace and Weerapana et al (2014) A competitive chemical-proteomic platform to identify zinc-binding cysteines. ACS Chem.Biol. 9 258 PMID: 24111988
Macpherson et al (2007) Noxious compounds activate TRPA1 ion channels through covalent modification of cysteines. Nature 445 541 PMID: 17237762
If you know of a relevant reference for IA-Alkyne, please let us know.
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