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JQ1-FITC

Cat. No. 7722 1 Citation Submit a Review Datasheet / COA / SDS
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Description: Fluorescent BET bromodomain probe; suitable for use in TR-FRET
Chemical Name: (S)-5-(3-(1-(4-(4-Chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-2-oxo-7,10,13-trioxa-3-azahexadecan-16-yl)thioureido)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid trifluoroacetate
Purity: ≥95% (HPLC)
Datasheet
Citations (1)
Reviews
Literature (2)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for JQ1-FITC

JQ1-FITC is a fluorescent BET bromodomain probe. It binds with high affinity to BRD4(BD1) and BRD4(BD2) (KD,app values are 6.5 nM and 5.8 nM, respectively, using GST-tagged BRD4 proteins measured by CoraFluor™ TR-FRET assay; 58.7 nM for BRD4(BD1) in a fluorescence anisotropy assay). Excitation and emission maxima (λ) are 495 nm and 525 nm.

Optical Data for JQ1-FITC

JQ1-FITC Dye Spectra
Emission Color Green
λabs 495 nm
λem 525 nm
Extinction Coefficient (ε) 7500 M-1cm-1
Quantum Yield (φ) 0.92
Closest Laser line 488 nm
Application TR-FRET, Fluorescence polarization assays
Alternative For FITC

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for JQ1-FITC

M. Wt 1106.58
Formula C50H50ClN7O9S2.CF3CO2H
Storage Store at -20°C
Purity ≥95% (HPLC)
InChI Key QRVVCZSOSIMDNN-LMDYQTDMSA-N
Smiles O=C(NCCCOCCOCCOCCCNC(NC1=CC=C(C(C(O)=O)=C1)C2=C(C(OC3=C2C=CC(O)=C3)=C4)C=CC4=O)=S)C[C@@H]5N=C(C6=CC=C(Cl)C=C6)C7=C(SC(C)=C7C)N8C5=NN=C8C.OC(C(F)(F)F)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for JQ1-FITC

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 11.07 10

Preparing Stock Solutions for JQ1-FITC

The following data is based on the product molecular weight 1106.58. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 9.04 mL 45.18 mL 90.37 mL
0.5 mM 1.81 mL 9.04 mL 18.07 mL
1 mM 0.9 mL 4.52 mL 9.04 mL
5 mM 0.18 mL 0.9 mL 1.81 mL

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References for JQ1-FITC

References are publications that support the biological activity of the product.

Payne et al (2022) A direct high-throughput protein quantification strategy facilitates discovery and characterization of a celastrol-derived BRD4 degrader. Cell Chem.Biol. 18 1333 PMID: 35649410

If you know of a relevant reference for JQ1-FITC, please let us know.

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1 Citation for JQ1-FITC

Citations are publications that use Tocris products. Selected citations for JQ1-FITC include:

Payne et al (2024) Protocol for the comprehensive biochemical and cellular profiling of small-molecule degraders using CoraFluor TR-FRET technology. STAR Protoc. 5 103129 PMID: 38857155

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Literature in this Area

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