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Submit ReviewProtiFect STAR™ Protein Transfection Reagent
Description: Protein transfection reagent
Biological Activity
ProtiFect STAR™ Protein Transfection Reagent is a transfection reagent capable of delivering a wide range of proteins with varying molecular weights (up to 240 kDa) and isoelectric points to the cytoplasm, including Cas9 ribonucleoproteins and BSA-FITC. ProtiFect STAR™ is a gemini amphiphile, which bypasses endocytic pathways. Can be used in vivo and in vitro in a variety of cell types including primary cells, T-cells, B-cells and stem cells. For more information on how to use ProtiFect STAR™, please see our protocol.
This compound is supplied pre-dissolved in ethanol (10 mg/mL).
Scientific Data
Application of ProtiFect STAR™ Protein Transfection Reagent. Intracellular delivery of negatively charged green fluorescence protein (GFP), superoxide dismutase (SOD), ovalbumin (OVA), and positively charged lysozyme with a commercially available protein transfection reagent and ProtiFect STAR™. Proteins and ProtiFect STAR™ were at 8 μg/mL (n = 3). Scale = 25 μm.
Application of ProtiFect STAR™ Protein Transfection Reagent. Intracellular delivery of CRISPR/Cas9 ribonucleoprotein complexes with ProtiFect STAR™ in 293T cells targeting adeno-associated virus integration site 1 (AAVS1), leading to an indel frequency of 37.8%.
Application of ProtiFect STAR™ Protein Transfection Reagent. Intracellular delivery of CRISPR/Cas9 ribonucleoprotein complexes with ProtiFect STAR™ in SW-480 cells targeting KRAS locus. CMAX was used as a positive control. Sanger sequence (lower panel) performed by T-A cloning at KRAS locus was obtained from SW-480 cells. Three sequences of clones with mutations were represented. Cas9 protein, sgRNA, and ProtiFect STAR™ were 2, 1, and 4 μg/mL, respectively.
Technical Data
| Storage | Store at -20°C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solubility | Soluble in ethanol (supplied pre-dissolved in anhydrous ethanol, 10mg/ml) |
Product Datasheets
References
References are publications that support the biological activity of the product.
Le et al (2023) Direct cytosolic delivery of proteins and CRISPR-Cas9 genome editing by gemini amphiphiles via non-endocytic translocation pathways. ACS Cent.Sci. 9 1313 PMID: 37521791
Zhao et al (2023) Gemini amphiphiles: robust cytosolic delivery of therapeutic biomacromolecules through non-endocytic translocation pathways. AC Cent.Sci. 9 1266 PMID: 37521797
If you know of a relevant reference for ProtiFect STAR™ Protein Transfection Reagent, please let us know.
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