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Submit ReviewSulfo-NHS-LC-Biotin
Description: Amine reactive biotinylation reagent, cell impermeable
Chemical Name: Sodium 2,5-dioxo-1-((6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanoyl)oxy)pyrrolidine-3-sulfonate
Biological Activity for Sulfo-NHS-LC-Biotin
Sulfo-NHS-LC-Biotin is a water-soluble amine reactive biotinylation reagent used to attach biotin to primary amines. Commonly used to label proteins and antibodies. Does not penetrate membrane. Reactive in pH 7.0-9.0, solubility 10 mg/mL in water and DMF.
Technical Data for Sulfo-NHS-LC-Biotin
| M. Wt | 556.58 |
| Formula | C20H29N4NaO9S2 |
| Storage | Store at -20°C |
| CAS Number | 191671-46-2 |
| PubChem ID | 71571497 |
| InChI Key | JJGWLCLUQNFDIS-GTSONSFRSA-M |
| Smiles | [H][C@]12CS[C@H]([C@]1(NC(N2)=O)[H])CCCCC(NCCCCCC(ON3C(CC(S([O-])(=O)=O)C3=O)=O)=O)=O.[Na+] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Sulfo-NHS-LC-Biotin
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 55.66 | 100 | |
| DMSO | 55.66 | 100 |
Preparing Stock Solutions for Sulfo-NHS-LC-Biotin
The following data is based on the product molecular weight 556.58. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.8 mL | 8.98 mL | 17.97 mL |
| 5 mM | 0.36 mL | 1.8 mL | 3.59 mL |
| 10 mM | 0.18 mL | 0.9 mL | 1.8 mL |
| 50 mM | 0.04 mL | 0.18 mL | 0.36 mL |
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Product Datasheets for Sulfo-NHS-LC-Biotin
References for Sulfo-NHS-LC-Biotin
References are publications that support the biological activity of the product.
Takayama et al (2017) Long-term drug modification to the surface of mesenchymal stem cells by the avidin-biotin complex method. Sci.Rep 7 16953 PMID: 29208980
Xu and Wegner (2020) Multifunctional streptavidin-biotin conjugates with precise stoichiometries. Chem.Sci. 11 4422
Karunakaran et al (2020) Butyrophilin-2A1 directly binds germline-encoded regions of the Vγ9Vδ2 TCR and is essential for phosphoantigen sensing. Immunity 52 487 PMID: 32155411
If you know of a relevant reference for Sulfo-NHS-LC-Biotin, please let us know.
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Keywords: Sulfo-NHS-LC-Biotin, Sulfo-NHS-LC-Biotin supplier, Water, soluble, biotinylation, reagents, primary, amine, labels, Biochemicals, and, Molecular, Biology, 7302, Tocris Bioscience
1 Citation for Sulfo-NHS-LC-Biotin
Citations are publications that use Tocris products. Selected citations for Sulfo-NHS-LC-Biotin include:
Andrei I et al (2023) Inflammation modulates intercellular adhesion and mechanotransduction in human epidermis via ROCK2. iScience 26 106195 PMID: 36890793
Do you know of a great paper that uses Sulfo-NHS-LC-Biotin from Tocris? Please let us know.
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