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Submit ReviewCoraFluor™ 1, amine reactive
Description: Terbium cryptate FRET donor for TR-FRET assay development; amine reactive for conjugation
Biological Activity for CoraFluor™ 1, amine reactive
CoraFluor™ 1, amine reactive is a terbium-based time-resolved Förster resonance energy transfer (TR-FRET) donor for TR-FRET assay development, such as target engagement and ternary complex assays. This product is designed with a PFP ester for reactivity with primary amines, making it suitable for labeling biomolecules, including antibodies, nanobodies, proteins and peptides. It is exceptionally bright, providing unmatched assay sensitivity and stability in biological media over other commercially available TR-FRET donors. CoraFluor™ 1, amine reactive is compatible with common acceptor dyes such as FAM, TMR and Cyanine 5, SE (Cat. No. 5436), enabling seamless assay integration in high throughput screening applications.
CoraFluor™ 1, thiol reactive and CoraFluor™ 2, amine reactive also available.
Please refer to the protocol for further information on conjugation.
CoraFluor™ is a trademark of Bio-Techne Corp.
Scientific Data
CoraFluor™ 1 stability profile. Stability profiling of CoraFluor™ 1 and a commercially available TR-FRET donor (5 nM) after 7 days of exposure to 50 mM PBS, acetic acid (pH 3), sodium acetate (pH 5), MES (pH 6) or Tris buffer (pH 7.5) supplemented with various additives (DTPA, Ca2+, Mg2+, DTT, TCEP = 1 mM; Mn2+, Cu2+ = 0.5 mM).
Binding Activity
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CoraFluor™ TR-FRET target engagement assay. High throughput TR-FRET target engagement assay profiling BRD4 inhibitors and Degraders on endogenous BRD4 using a single detection mix consisting of a primary BRD4 antibody, a CoraFluor™ 1 labeled VHH secondary antibody, and a fluorescent JQ1-based tracer (JQ1-FITC Catalog #7722).
Licensing Information
Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
Optical Data for CoraFluor™ 1, amine reactive
| λabs | 338 nm |
|---|---|
| Extinction Coefficient (ε) | 23100 M-1cm-1 |
| Quantum Yield (φ) | 0.58 |
| Closest Laser line | 337 nm |
| Reactive Group | PFP ester |
| Reactivity | Primary amines |
| Correction Factor 280 | 0.157 |
| Application | Time-resolved fluorescence energy transfer (TR-FRET) |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for CoraFluor™ 1, amine reactive
| M. Wt | 1485.25 |
| Storage | Store at -20°C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for CoraFluor™ 1, amine reactive
References for CoraFluor™ 1, amine reactive
References are publications that support the biological activity of the product.
Payne et al (2021) Bright and stable luminescent probes for target engagement profiling in live cells. Nat.Chem.Biol. 17 1168 PMID: 34675420
Payne et al (2022) A direct high-throughput protein quantification strategy facilitates discovery and characterization of a celastrol-derived BRD4 degrader. Cell Chem.Biol. 29 1333 PMID: 35649410
Payne et al (2022) Resolving the deceptive isoform and complex selectivity of HDAC1/2 inhibitors. Cell Chem.Biol. 29 1140 PMID: 35298895
If you know of a relevant reference for CoraFluor™ 1, amine reactive, please let us know.
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1 Citation for CoraFluor™ 1, amine reactive
Citations are publications that use Tocris products. Selected citations for CoraFluor™ 1, amine reactive include:
Payne et al (2024) Protocol for the comprehensive biochemical and cellular profiling of small-molecule degraders using CoraFluor TR-FRET technology. STAR Protoc. 5 103129 PMID: 38857155
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Protocols for CoraFluor™ 1, amine reactive
The following protocol features additional information for the use of CoraFluor™ 1, amine reactive (Cat. No. 7920).
Literature in this Area
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