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Submit ReviewCoraFluor™ 2, amine reactive
Description: Terbium cryptate FRET donor for TR-FRET assay development; amine reactive for conjugation
Biological Activity for CoraFluor™ 2, amine reactive
CoraFluor™ 2, amine reactive is a terbium-based time-resolved Förster resonance energy transfer (TR-FRET) donor for TR-FRET assay development, such as target engagement and ternary complex assays. CoraFluor™ 2 is designed with a PFP ester for reactivity with primary amines, making it suitable for labeling biomolecules, including antibodies, nanobodies, proteins and peptides. CoraFluor™ 2 is cell permeable and can be used in live cell intracellular TR-FRET assays. It is exceptionally bright, providing unmatched assay sensitivity and stability in biological media over other commercially available TR-FRET donors. CoraFluor™ 2 displays red shifted excitation and is excitable by both 365 nm and 405 nm lasers, and is compatible with common acceptor dyes such as FAM/FITC, AF488, GFP and mCherry, enabling seamless assay integration in high throughput screening applications.
CoraFluor™ 1, amine reactive and CoraFluor™ 1, thiol reactive also available.
Please refer to the protocol for further information on conjugation.
CoraFluor™ is a trademark of Bio-Techne Corp.
Scientific Data
CoraFluor™ 2 stability profile. Stability profiling of CoraFluor™ 2 and a commercially available TR-FRET donor (5 nM) after 7 days of exposure to 50 mM PBS, acetic acid (pH 3), sodium acetate (pH 5), MES (pH 6) or Tris buffer (pH 7.5) supplemented with various additives (DTPA, Ca2+, Mg2+, DTT, TCEP = 1 mM; Mn2+, Cu2+ = 0.5 mM).
Binding Activity
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Live cell TR-FRET assay. Cellular dose-dependent inhibition of HDAC1 via CoraFluor™ 2 labeled HaloTag® in HEK293T cells by various HDAC inhibitors (treatment for 4 h at 37 °C, 1 μM SAHA-NCT). HaloTag is a trademark of Promega Corporation.
Licensing Information
Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
Optical Data for CoraFluor™ 2, amine reactive
| λabs | 352 nm |
|---|---|
| Reactivity | Primary amines |
| Application | Time-resolved fluorescence energy transfer (TR-FRET) |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for CoraFluor™ 2, amine reactive
| M. Wt | 1623.02 |
| Storage | Store at -20°C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for CoraFluor™ 2, amine reactive
References for CoraFluor™ 2, amine reactive
References are publications that support the biological activity of the product.
Payne et al (2021) Bright and stable luminescent probes for target engagement profiling in live cells. Nat.Chem.Biol. 17 1168 PMID: 34675420
If you know of a relevant reference for CoraFluor™ 2, amine reactive, please let us know.
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Protocols for CoraFluor™ 2, amine reactive
The following protocol features additional information for the use of CoraFluor™ 2, amine reactive (Cat. No. 7950).
Literature in this Area
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